枯草芽孢杆菌苏氨酸tRNA的核苷酸序列。
Nucleotide sequence of threonine tRNA from Bacillus subtilis.
作者信息
Hasegawa T, Ishikura H
出版信息
Nucleic Acids Res. 1978 Feb;5(2):537-48. doi: 10.1093/nar/5.2.537.
Abstract
A threonine tRNA was purified from Bacillus subtilis W168 by a combined use of column chromatographic systems. The nucleotide sequence was determined to be pG-C-C-G-G-U-G-U-A-G-C-U-C-A-A-U-D-G-G-D(U)-A-G-A-G-C-A-A-C-U-G-A-C-U-mo5U-G-U-t6A-A-psi-C-A-G-U-A-G-m7G-U-U-G-G-G-G-G-T-psi-C-A-A-G-U-C-C-U-C-U-U-G-C-C-G-G-C-A-C-C-AOH, where about 40 % of D20 remained unmodified as U20. It consists of 76 nucleotides including a new minor nucleoside, 5-methoxyuridine (mo5U), which occupies the wobble position of anticodon.
摘要
通过联用柱色谱系统从枯草芽孢杆菌W168中纯化出一种苏氨酸tRNA。测定其核苷酸序列为pG-C-C-G-G-U-G-U-A-G-C-U-C-A-A-U-D-G-G-D(U)-A-G-A-G-C-A-A-C-U-G-A-C-U-mo5U-G-U-t6A-A-psi-C-A-G-U-A-G-m7G-U-U-G-G-G-G-G-T-psi-C-A-A-G-U-C-C-U-C-U-U-G-C-C-G-G-C-A-C-C-AOH，其中约40%的D20未被修饰而仍为U20。它由76个核苷酸组成，包括一种新的稀有核苷5-甲氧基尿苷（mo5U），其占据反密码子的摆动位置。
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