Department of Biochemistry, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, Tamilnadu, India.
Department of Animal Physiology and Biochemistry, National Animal Resource Facility for Biomedical Research (ICMR-NARFBR), Hyderabad, Telangana, India.
J Cardiovasc Pharmacol. 2022 Jan 1;79(1):e75-e86. doi: 10.1097/FJC.0000000000001167.
The present study was intended to evaluate the effect of polyherbal formulation (PHF) made with 3 nutraceuticals, such as Piper nigrum, Terminalia paniculata, and Bauhinia purpurea on inflammation and oxidative stress in diabetic cardiomyopathy (DCM), which is induced by streptozotocin and nicotinamide administration in rats. We supplemented DCM rats with PHF (250 and 500 mg/kg/BW) for 45 days and evaluated their effects on oxidative stress markers, proinflammatory cytokines, and messenger RNA expressions of the nuclear factor erythroid 2-related factor-2 (Nrf-2) and its linked genes [heme oxygenase-1 (HO-1), superoxide dismutase, catalase] along with inflammatory genes [tumour necrosis factor α and nuclear factor kappa B (NF-κB)]. Our study demonstrated that PHF successfully attenuated inflammation and oxidative stress via messenger RNA upregulation of Nrf-2, HO-1, superoxide dismutase, and catalase and concomitantly with downregulation of tumour necrosis factor α and NF-κB. Conversely, PHF also protected hyperglycemia-mediated cardiac damage, which was confirmed with histopathological and scanning electron microscopy analysis. In conclusion, our results suggested that PHF successfully ameliorated hyperglycemia-mediated inflammation and oxidative stress via regulation of NF-κB/Nrf-2/HO-1 pathway. Therefore, these results recommend that PHF may be a prospective therapeutic agent for DCM.
本研究旨在评估由三种营养保健品（黑胡椒、诃子和紫荆）组成的复方草药制剂（PHF）对链脲佐菌素和烟酰胺给药诱导的糖尿病心肌病（DCM）中炎症和氧化应激的影响。我们用 PHF（250 和 500 mg/kg/BW）对 DCM 大鼠进行了 45 天的补充，并评估了其对氧化应激标志物、促炎细胞因子、核因子红细胞 2 相关因子 2（Nrf-2）及其相关基因[血红素加氧酶-1（HO-1）、超氧化物歧化酶、过氧化氢酶]和炎症基因[肿瘤坏死因子α和核因子 kappa B（NF-κB）]的信使 RNA 表达的影响。我们的研究表明，PHF 通过信使 RNA 上调 Nrf-2、HO-1、超氧化物歧化酶和过氧化氢酶，同时下调肿瘤坏死因子α和 NF-κB，成功减轻了炎症和氧化应激。相反，PHF 还通过组织病理学和扫描电子显微镜分析保护了高血糖介导的心脏损伤。总之，我们的结果表明，PHF 通过调节 NF-κB/Nrf-2/HO-1 通路成功改善了高血糖介导的炎症和氧化应激。因此，这些结果表明 PHF 可能是 DCM 的一种有前途的治疗药物。
