Blick M, Romero P, Talpaz M, Kurzrock R, Shtalrid M, Andersson B, Trujillo J, Beran M, Gutterman J
Cancer Genet Cytogenet. 1987 Aug;27(2):349-56. doi: 10.1016/0165-4608(87)90018-5.
We have studied the expression of c-abl and c-myc in leukemic cells of patients in all clinical phases of chronic myelogenous leukemia. We demonstrate that an aberrant 8-Kb c-abl related transcript is present in the RNA of the leukemic cell from all patients with Ph+ CML and that the loss of both normal chromosome #9 is associated with the loss of the normal c-abl related transcripts. This represents direct evidence that the normal c-abl related transcripts derive from the normal c-abl gene locus on the normal chromosome #9, while the aberrant c-abl related transcript in Ph+ CML derives from the hybrid bcr-abl gene formed as a result of the t(9;22). We further demonstrate that trisomy 8 in some instances is associated with enhanced expression of the c-myc oncogene.
我们研究了慢性粒细胞白血病所有临床阶段患者白血病细胞中c-abl和c-myc的表达。我们证明，在所有Ph+慢性粒细胞白血病患者的白血病细胞RNA中都存在一种异常的8千碱基c-abl相关转录本，并且两条正常9号染色体的缺失与正常c-abl相关转录本的缺失相关。这代表了直接证据，即正常c-abl相关转录本来自正常9号染色体上的正常c-abl基因位点，而Ph+慢性粒细胞白血病中的异常c-abl相关转录本来自因t(9;22)形成的融合bcr-abl基因。我们进一步证明，在某些情况下，8号染色体三体与c-myc癌基因的表达增强相关。
