School of Chemistry and Environment, College of Food Science and Technology, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang, 524088, China.
School of Chemistry and Environment, College of Food Science and Technology, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Zhanjiang, 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang, 524025, China.
Chem Biol Interact. 2022 Jan 5;351:109737. doi: 10.1016/j.cbi.2021.109737. Epub 2021 Nov 2.
Excessive exposure to Ultraviolet (UV) rays can cause premature skin aging. Ishigoside (IGS) is a new glyceroglycolipid compound isolated from brown algal Ishige okamurae, However, whether it can protect the skin from (Ultraviolet-B) UVB damage has not been illuminated.
The in vitro anti-photoaging effect of IGS was conducted in UVB-induced HaCaT. The HaCaT cells were divided into the following five groups: (1) cells didn't suffer from UVB irradiation or IGS treatment. (2-5) Cells were treated with various concentrations of IGS (0, 10, 50, and 100 μM) and irradiated by 40 mJ/cm UVB. The Matrix metalloproteinase (MMP) of photoaging process was determined by ELISA kits and the latent interaction between IGS and MMP was further performed by molecular docking. The crucial signaling pathway proteins involved in the collagen synthesis and degradation were subsequently evaluated by Western blotting, immunofluorescence and EMSA.
IGS effectively suppresses the high expressions and secretions of matrix metalloproteinases (MMPs) and photo-inflammation by blocking MAPKs, AP-1 and NF-κB. Meanwhile, increasing antioxidant enzyme expression. Molecular docking results suggest that inhibition of IGS on MMPs may be attributed to its hydrogen supply and hydrophobic capacity. In addition, IGS enhanced procollagen production by upregulating the TGF-β/Smad pathways.
IGS exhibited anti-photoaging activity in UVB-damage HaCaT. These effects might be a contribution by its suppression of MMPs expression via MAPKs, AP-1 and NF-κB pathway and have anti-oxidative and anti-inflammatory effects. Therefore, IGS has the great potential to become skin-care products or functional foods for preventing skin photoaging.
过度暴露在紫外线下（UV）会导致皮肤过早老化。石仙桃苷（IGS）是从褐藻石仙桃中分离得到的一种新的甘油糖脂化合物，然而，它是否能保护皮肤免受（紫外线-B）UVB 损伤尚未得到阐明。
在 UVB 诱导的 HaCaT 中进行 IGS 的体外抗光老化作用研究。将 HaCaT 细胞分为以下五组：（1）细胞未遭受 UVB 照射或 IGS 处理。（2-5）细胞用不同浓度的 IGS（0、10、50 和 100 μM）处理，并接受 40 mJ/cm UVB 照射。通过 ELISA 试剂盒测定光老化过程中的基质金属蛋白酶（MMP），并通过分子对接进一步研究 IGS 与 MMP 之间的潜在相互作用。随后通过 Western blot、免疫荧光和 EMSA 评估参与胶原合成和降解的关键信号通路蛋白。
IGS 通过阻断 MAPKs、AP-1 和 NF-κB，有效抑制基质金属蛋白酶（MMPs）和光炎症的高表达和分泌。同时，增加抗氧化酶的表达。分子对接结果表明，IGS 对 MMPs 的抑制可能归因于其供氢和疏水性。此外，IGS 通过上调 TGF-β/Smad 通路增强原胶原蛋白的产生。
IGS 在 UVB 损伤的 HaCaT 中表现出抗光老化活性。这些作用可能是通过其抑制 MAPKs、AP-1 和 NF-κB 通路的 MMPs 表达，以及具有抗氧化和抗炎作用所致。因此，IGS 具有成为预防皮肤光老化的皮肤护理产品或功能性食品的巨大潜力。
