Department of Anatomy, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand.
Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi, Bang Mod, Bangkok 10140, Thailand.
Mar Drugs. 2022 Nov 11;20(11):707. doi: 10.3390/md20110707.
is a red alga distributed along the coasts of Southeast Asian countries including Thailand. Previous studies have shown that an ethyl acetate fraction of (HDEA), containing major compounds including n-hexadecanoic acid, 2-butyl-5-hexyloctahydro-1H-indene, 3-(hydroxyacetyl) indole and indole-3-carboxylic acid, possesses high antioxidant and anti-lung cancer activities. The present study demonstrated that HDEA could protect mouse skin fibroblasts (L929) and human immortalized keratinocytes (HaCaT) against photoaging due to ultraviolet A and B (UVA and UVB) by reducing intracellular reactive oxygen species (ROS) and expressions of matrix metalloproteinases (MMP1 and MMP3), as well as increasing Nrf2 nuclear translocation, upregulations of mRNA transcripts of antioxidant enzymes, including superoxide dismutase (), heme oxygenase () and glutathione S-transferase pi1 (), and procollagen synthesis. The results indicate that HDEA has the potential to protect skin cells from UV irradiation through the activation of the Nrf2 pathway, which leads to decreasing intracellular ROS and MMP production, along with the restoration of skin collagen.
是一种分布在东南亚国家（包括泰国）沿海的红藻。先前的研究表明，（HDEA）的乙酸乙酯部分含有主要化合物，包括正十六烷酸、2-丁基-5-己基-八氢-1H-茚、3-（羟乙酰基）吲哚和吲哚-3-羧酸，具有高抗氧化和抗肺癌活性。本研究表明，HDEA 通过减少细胞内活性氧物种（ROS）和基质金属蛋白酶（MMP1 和 MMP3）的表达，以及增加 Nrf2 核易位、上调抗氧化酶的 mRNA 转录物，包括超氧化物歧化酶（）、血红素加氧酶（）和谷胱甘肽 S-转移酶 pi1（）和前胶原蛋白的合成，可防止由于紫外线 A 和 B（UVA 和 UVB）引起的小鼠皮肤成纤维细胞（L929）和人永生化角质形成细胞（HaCaT）的光老化。结果表明，HDEA 通过激活 Nrf2 途径，减少细胞内 ROS 和 MMP 的产生，同时恢复皮肤胶原蛋白，从而具有保护皮肤细胞免受紫外线照射的潜力。
