Wu Di, Qu Fengting, Li Dan, Zhao Yue, Li Xiang, Niu Sijie, Zhao Maoyuan, Qi Haishi, Wei Zimin, Song Caihong
College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China; College of Life Science, Northeast Agricultural University, Harbin 150030, China.
College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Sci Total Environ. 2022 Feb 1;806(Pt 4):151376. doi: 10.1016/j.scitotenv.2021.151376. Epub 2021 Nov 2.
The aims of this article were to study the effect of Fenton pretreatment and bacterial inoculation on cellulose-degrading genes and fungal communities during rice straw composting. The rice straw was pretreated by Fenton reactions and functional bacterial agents were then inoculated during the cooling phase of composting. Three treatment groups were carried out, the control (CK), Fenton pretreatment (FeW) and Fenton pretreatment and bacterial inoculation (FeWI). The results indicated that Fenton pretreatment and bacterial inoculation changed the fungal communities composition and increased fungal diversity, leading to changes in the cellulose-degrading genes. In addition, a network analysis showed that in the FeWI treatment, the fungi from modules 1, 5 and 8 were core hosts of the cellulose-degrading genes driving the cellulosic degradation. Moreover, Fenton pretreatment and bacterial inoculation changed the core module fungal communities and strengthened the correlation between the core fungi and the cellulose-degrading genes, thereby promoting cellulosic degradation. Based on redundancy and structural equation model analyses, the NH-N, TOC, pH and Shannon index were important factors influencing the variations in the cellulose-degrading genes. This study provides a foundation for cellulosic degradation during cellulosic waste composting.
本文旨在研究芬顿预处理和接种细菌对稻草堆肥过程中纤维素降解基因和真菌群落的影响。通过芬顿反应对稻草进行预处理，然后在堆肥冷却阶段接种功能性细菌制剂。设置了三个处理组，分别为对照组（CK）、芬顿预处理组（FeW）和芬顿预处理并接种细菌组（FeWI）。结果表明，芬顿预处理和接种细菌改变了真菌群落组成，增加了真菌多样性，进而导致纤维素降解基因发生变化。此外，网络分析表明，在FeWI处理中，来自模块1、5和8的真菌是驱动纤维素降解的纤维素降解基因的核心宿主。而且，芬顿预处理和接种细菌改变了核心模块真菌群落，加强了核心真菌与纤维素降解基因之间的相关性，从而促进了纤维素降解。基于冗余分析和结构方程模型分析，铵态氮、总有机碳、pH值和香农指数是影响纤维素降解基因变异的重要因素。本研究为纤维素类废弃物堆肥过程中的纤维素降解提供了依据。
