过敏性鼻炎患者肠道微生物菌群多样性和组成的改变。
Altered diversity and composition of gut microbiota in patients with allergic rhinitis.
机构信息
Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, Hunan, China.
Shanghai Clinical Research Center, Shanghai, China.
出版信息
Microb Pathog. 2021 Dec;161(Pt A):105272. doi: 10.1016/j.micpath.2021.105272. Epub 2021 Nov 2.
BACKGROUND
Recently, multiple studies have suggested an association between gut dysbiosis and allergic rhinitis (AR) development. However, the role of gut microbiota in AR development remains obscure.
METHODS
The goal of this study was to compare the gut microbiota composition and short-chain fatty acid (SCFAs) differences associated with AR (N = 18) and HCs (healthy controls, N = 17). Gut microbiota 16SrRNA gene sequences were analyzed based on next-generation sequencing. SCFAs in stool samples were analyzed by gas chromatography-mass spectrometry (GC-MS).
RESULTS
Compared with HCs, the gut microbiota composition of AR was significantly different in diversity and richness. At the phylum level, the abundance of Firmicutes in the AR group were significantly lower than those in the HCs group. At the genus level, the abundance of Blautia, Eubacterium_hallii_group, Romboutsia, Collinsella, Dorea, Subdoligranulum and Fusicatenibacter in the AR group were significantly lower than that in the HCs group. The concentrations of SCFAs were significantly lower in the AR group compared with the HCs group. Correlation analysis showed that the Eubacterium-hallii-group and Blautia correlated positively with SCFAs.
CONCLUSION
Our results demonstrate compositional and functional alterations of the gut microbiome in AR.
背景
最近，多项研究表明肠道菌群失调与过敏性鼻炎（AR）的发展有关。然而，肠道微生物群在 AR 发展中的作用仍不清楚。
方法
本研究旨在比较 AR（N=18）和 HC（健康对照，N=17）患者肠道微生物群落组成和短链脂肪酸（SCFAs）的差异。基于下一代测序分析肠道微生物 16SrRNA 基因序列。通过气相色谱-质谱（GC-MS）分析粪便样本中的 SCFAs。
结果
与 HC 相比，AR 患者的肠道微生物群落组成在多样性和丰富度方面存在显著差异。在门水平上，AR 组厚壁菌门的丰度明显低于 HC 组。在属水平上，AR 组中 Blautia、Eubacterium_hallii_group、Romboutsia、Collinsella、Dorea、Subdoligranulum 和 Fusicatenibacter 的丰度明显低于 HC 组。AR 组的 SCFAs 浓度明显低于 HC 组。相关性分析表明，Eubacterium-hallii_group 和 Blautia 与 SCFAs 呈正相关。
结论
我们的研究结果表明 AR 患者的肠道微生物群存在组成和功能改变。
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