5-氨基酮戊酸光动力疗法对尖锐湿疣角质形成细胞中 Toll 样受体 4 和核因子 kappa B 表达的影响。
Effect of 5-aminolevulinic acid photodynamic therapy on the expression of Toll-like receptor 4 and nuclear factor kappa B in condyloma acuminatum keratinocytes.
机构信息
The First Affiliated Hospital of Zhengzhou University, China.
The First Affiliated Hospital of Zhengzhou University, China.
出版信息
Photodiagnosis Photodyn Ther. 2022 Mar;37:102614. doi: 10.1016/j.pdpdt.2021.102614. Epub 2021 Nov 3.
BACKGROUND
Condyloma acuminatum (CA) is a common sexually transmitted disease caused by human papillomaviruses. Five-aminolevulinic acid-photodynamic therapy (ALA-PDT) can promote the apoptosis of keratinocytes and inhibit proliferation; however, the effect of ALA-PDT on the immune mechanism of CA tissue is not well understood. In this study, we examined the changes in Toll-like receptor 4 (TRL4) and nuclear factor kappa B (NF-κB) expression in CA tissues before ALA-PDT treatment to determine its effects and possible immune mechanisms.
METHODS
Immunohistochemistry (streptavidin-peroxidase) was used to detect the expression of TLR4 and NF-κB in the keratinocytes of the patients with CA before and after ALA-PDT treatment.
RESULTS
Before treatment, the positive TLR4 and NF-κB expression rates in the keratinocytes of the patients with CA were 86.53% (45/52) and 94.23% (49/52), respectively, whereas after treatment, these rates were 44.23% (23/52) and 38.46% (20/52), respectively. Positive TLR4 and NF-κB expression in the keratinocytes of CA was mostly ++ to +++ in intensity before ALA-PDT and mostly ranged from - to + after treatment. The positive expression rate and intensity of TLR4 and NF-κB in the two groups before and after ALA-PDT were significantly different (P < 0.05). There was a positive correlation between the expression of TLR4 and NF-κB in the CA tissues after ALA-PDT (r = 0.486, P < 0.05).
CONCLUSIONS
ALA-PDT may relieve local immunosuppressive states by reducing TLR4 and NF-κB expression and jointly promoting CA regression, which is a potential molecular mechanism of ALA-PDT in CA treatment.
背景
尖锐湿疣（CA）是一种由人乳头瘤病毒引起的常见性传播疾病。5-氨基酮戊酸光动力疗法（ALA-PDT）可以促进角质形成细胞凋亡和抑制增殖；然而，ALA-PDT 对 CA 组织免疫机制的影响尚不清楚。在本研究中，我们检测了 ALA-PDT 治疗前 CA 组织中 Toll 样受体 4（TLR4）和核因子 kappa B（NF-κB）表达的变化，以确定其作用及其可能的免疫机制。
方法
采用免疫组织化学（链霉亲和素-过氧化物酶）检测 ALA-PDT 治疗前后 CA 患者角质形成细胞中 TLR4 和 NF-κB 的表达。
结果
治疗前，CA 患者角质形成细胞中 TLR4 和 NF-κB 的阳性表达率分别为 86.53%（45/52）和 94.23%（49/52），治疗后分别为 44.23%（23/52）和 38.46%（20/52）。ALA-PDT 治疗前，CA 角质形成细胞中 TLR4 和 NF-κB 的阳性表达强度大多为++至+++，治疗后大多为-至+。ALA-PDT 前后两组 TLR4 和 NF-κB 的阳性表达率和强度差异均有统计学意义（P<0.05）。ALA-PDT 后 CA 组织中 TLR4 和 NF-κB 的表达呈正相关（r=0.486，P<0.05）。
结论
ALA-PDT 通过降低 TLR4 和 NF-κB 的表达，共同促进 CA 消退，可能缓解局部免疫抑制状态，这可能是 ALA-PDT 治疗 CA 的潜在分子机制。
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