The First Affiliated Hospital of Zhengzhou University in China, China.
The First Affiliated Hospital of Zhengzhou University in China, China.
Photodiagnosis Photodyn Ther. 2019 Dec;28:53-57. doi: 10.1016/j.pdpdt.2019.07.018. Epub 2019 Jul 23.
Condyloma acuminatum is a highly infectious disease caused by the human papillomavirus. Previous studies have shown that 5-aminolevulinic acid photodynamic therapy can inhibit proliferation of condyloma acuminatum keratinocytes. However, the effect of 5-aminolevulinic acid photodynamic therapy on condyloma acuminatum tissues has not been systematically studied. Here, we investigated possible molecular mechanisms of 5-aminolevulinic acid photodynamic therapy in the treatment of condyloma acuminatum and its effect on the expression of apoptosis inhibitors Bcl-2 and Survivin.
Immunohistochemistry streptavidin-peroxidase was used to detect the expression of apoptosis inhibitors Bcl-2 and Survivin in condyloma acuminatum keratinocytes before and after the therapy.
The positive expression rates of Bcl-2 and Survivin in condyloma acuminatum keratinocytes before treatment were 87.50% (42/48) and 79.16% (38/48), respectively. The positive expression rates of Bcl-2 and Survivin in condyloma acuminatum keratinocytes after treatment were 37.50% (18/48) and 41.67% (20/48), respectively. The positive expression intensity of Bcl-2 and Survivin in condyloma acuminatum keratinocytes before 5-aminolevulinic acid photodynamic therapy was mostly ++ to +++, and that after treatment was mostly - to +. There were statistically significant differences in the positive expression rate and the expression intensity of Bcl-2 and Survivin in the two groups before and after 5-aminolevulinic acid photodynamic therapy (P < 0.001). There was a positive correlation between the expression of Bcl-2 and Survivin in condyloma acuminatum tissues after 5-aminolevulinic acid photodynamic therapy (r = 0.480, P < 0.05).
5-aminolevulinic acid photodynamic therapy may promote apoptosis of condyloma acuminatum cells by reducing the expression of Bcl-2 and Survivin, suggesting that this is potentially one of the molecular mechanisms of 5-aminolevulinic acid photodynamic therapy in the treatment of condyloma acuminatum.
尖锐湿疣是由人乳头瘤病毒引起的一种高度传染性疾病。先前的研究表明，5-氨基酮戊酸光动力疗法可以抑制尖锐湿疣角质形成细胞的增殖。然而，5-氨基酮戊酸光动力疗法对尖锐湿疣组织的影响尚未得到系统研究。在这里，我们研究了 5-氨基酮戊酸光动力疗法治疗尖锐湿疣的可能分子机制及其对凋亡抑制剂 Bcl-2 和 Survivin 表达的影响。
采用免疫组织化学链霉亲和素-过氧化物酶法检测治疗前后尖锐湿疣角质形成细胞中凋亡抑制剂 Bcl-2 和 Survivin 的表达。
治疗前尖锐湿疣角质形成细胞中 Bcl-2 和 Survivin 的阳性表达率分别为 87.50%（42/48）和 79.16%（38/48）。治疗后尖锐湿疣角质形成细胞中 Bcl-2 和 Survivin 的阳性表达率分别为 37.50%（18/48）和 41.67%（20/48）。5-氨基酮戊酸光动力疗法前尖锐湿疣角质形成细胞中 Bcl-2 和 Survivin 的阳性表达强度多为++至+++，治疗后多为-至+。5-氨基酮戊酸光动力疗法前后两组 Bcl-2 和 Survivin 的阳性表达率和表达强度差异均有统计学意义（P<0.001）。5-氨基酮戊酸光动力疗法后尖锐湿疣组织中 Bcl-2 和 Survivin 的表达呈正相关（r=0.480，P<0.05）。
5-氨基酮戊酸光动力疗法可能通过降低 Bcl-2 和 Survivin 的表达促进尖锐湿疣细胞凋亡，提示这可能是 5-氨基酮戊酸光动力疗法治疗尖锐湿疣的潜在分子机制之一。
