Sharples K, Plowman G D, Rose T M, Twardzik D R, Purchio A F
DNA. 1987 Jun;6(3):239-44. doi: 10.1089/dna.1987.6.239.
We have isolated a cDNA clone coding for the simian TGF-beta precursor from a cDNA library made from an African green monkey cell line, BSC-40. The deduced amino acid sequence of the mature simian TGF-beta shows 100% homology with that of the human TGF-beta: Strong sequence homology was found between the precursor regions of the human and simian proteins with only five amino acid changes out of 278. The simian (and murine) precursor sequence was found to code for one less amino acid residue than the human. The implications of these results with respect to the isolation of a growth inhibitor from these cells functionally related to the TGF-beta molecule are discussed.
我们从非洲绿猴细胞系BSC - 40构建的cDNA文库中分离出了编码猿猴转化生长因子β（TGF - β）前体的cDNA克隆。成熟猿猴TGF - β的推导氨基酸序列与人类TGF - β的氨基酸序列显示出100%的同源性：在人类和猿猴蛋白质的前体区域之间发现了很强的序列同源性，在278个氨基酸中只有5个氨基酸发生了变化。发现猿猴（和小鼠）的前体序列编码的氨基酸残基比人类少一个。讨论了这些结果对于从这些细胞中分离出与TGF - β分子功能相关的生长抑制剂的意义。
