Li Changsheng, Yuan Shankui, Zhou Yanming, Li Xuefeng, Duan Liusheng, Huang Lan, Zhou Xiaojin, Ma Yongqiang, Pang Sen
Department of Applied Chemistry, College of Sciences, China Agricultural University, Beijing 100193, China; Engineering Research Center of Plant Growth Regulator, Ministry of Education & College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100125, China.
Sci Total Environ. 2022 Feb 1;806(Pt 4):151475. doi: 10.1016/j.scitotenv.2021.151475. Epub 2021 Nov 4.
Microplastics (MPs) and pesticides are typical representatives of harmful chemicals in polluted waters. It is understood that the combined toxicity may differ from that of a single toxic substance. Although their combined toxicities on aquatic organisms have practical significance and research value, they have received little attention due to their complicated interaction, and the mechanism has rarely been reported. In this paper, we designed a study to investigate the single and combined effects of polystyrene microplastics (PS-MPs) and the triazole fungicide difenoconazole on zebrafish, and to explore the mechanism of this effect. The results showed that PS-MPs could reduce the bioaccumulation of difenoconazole in zebrafish to a certain extent and alleviate the oxidative stress damage of difenoconazole in the zebrafish liver. The transcriptome and qRT-PCR data revealed the association of multiple pathways in the difenoconazole response, while the presence of PS-MPs ameliorated this effect in gene expression changes. Due to the properties of PS-MPs and the interaction between them, the toxic effect of difenoconazole when combined with PS-MPs is more prominent. These results provide a novel aspect to understand the environmental behavior of MPs and to evaluate the combined effect of MPs and pesticides on aquatic food.
微塑料（MPs）和农药是污染水体中有害化学物质的典型代表。据了解，其联合毒性可能与单一有毒物质的毒性不同。尽管它们对水生生物的联合毒性具有实际意义和研究价值，但由于其复杂的相互作用，一直未受到足够关注，相关作用机制也鲜有报道。本文设计了一项研究，以调查聚苯乙烯微塑料（PS-MPs）和三唑类杀菌剂苯醚甲环唑对斑马鱼的单一和联合效应，并探究其作用机制。结果表明，PS-MPs在一定程度上可降低苯醚甲环唑在斑马鱼体内的生物累积，并减轻苯醚甲环唑对斑马鱼肝脏的氧化应激损伤。转录组和qRT-PCR数据揭示了苯醚甲环唑反应中多条途径的关联，而PS-MPs的存在改善了基因表达变化中的这种效应。由于PS-MPs的特性及其之间的相互作用，苯醚甲环唑与PS-MPs联合时的毒性作用更为突出。这些结果为理解微塑料的环境行为以及评估微塑料和农药对水生生物的联合效应提供了新的视角。
