Gao Fang-Zhou, He Liang-Ying, Hu Li-Xin, Chen Jun, Yang Yuan-Yuan, Zou Hai-Yan, He Lu-Xi, Bai Hong, Liu You-Sheng, Zhao Jian-Liang, Ying Guang-Guo
SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China.
Pearl River Water Resource Research Institute, Guangzhou 510611, China.
Sci Total Environ. 2022 Feb 1;806(Pt 4):151476. doi: 10.1016/j.scitotenv.2021.151476. Epub 2021 Nov 4.
Anthropogenic activities have inevitably impacted riverine ecosystems, yet their overall contribution to the assemblage of bacterial communities at a large river basin scale remains unclear. In this study, 16S amplicon sequencing was implemented to investigate the bacterial ecosystems in paired water and sediment of North River and West River basins in South China., which contains various anthropogenic environments (e.g., rural/urban area, mining area and livestock area). Subsequently, the links between bacterial community and various types of emerging pollutants in river water were analyzed. The results show that the bacterial assemblage of water and sediment had their own properties that the bacterial community of sediment were mainly affected by seasonal properties, while the bacterial community of water were affected by both seasons and anthropogenic activities. Therein, the aquatic bacterial compositions and abundances were driven by changes in temperature, dissolved oxygen and the emerging pollutants. The dominant phyla Proteobacteria and Firmicutes exhibited adaptability to the mining-affected regions, therein many clades (e.g., Beijerinckiaceae, Acetobacteraceae and Mycobacteriaceae) were also prevalent in the livestock-affected and densely-populated regions. In addition, these two phyla presented associations to the antibiotic resistance in water. The levels of antibiotics, relative antibiotic resistance gens (ARGs) and non-antibiotic pharmaceuticals (NAPs) were closely related to bacterial community composition, diversity and functional diversity, indicating their drive in shifting bacterial communities. Collectively, this work provides a basis for understanding the contribution of anthropogenic activities in shifting bacterial community at a large river basin scale. Further, the results provide new insights for expansion of ecological assessment.
人为活动不可避免地对河流生态系统产生了影响，但其在大河流域尺度上对细菌群落组合的总体贡献仍不清楚。在本研究中，采用16S扩增子测序技术对中国南方北江和西江流域成对的水体和沉积物中的细菌生态系统进行了调查，这些流域包含各种人为环境（如农村/城市地区、矿区和畜牧区）。随后，分析了细菌群落与河水中各种新型污染物之间的联系。结果表明，水体和沉积物中的细菌组合具有各自的特性，沉积物中的细菌群落主要受季节特性影响，而水体中的细菌群落则受季节和人为活动的共同影响。其中，水生细菌的组成和丰度受温度、溶解氧和新型污染物变化的驱动。优势菌门变形菌门和厚壁菌门对受采矿影响的区域表现出适应性，其中许多分支（如拜叶林克氏菌科、醋杆菌科和分枝杆菌科）在受畜牧影响和人口密集的区域也很普遍。此外，这两个菌门与水中的抗生素抗性有关。抗生素、相对抗生素抗性基因（ARGs）和非抗生素药物（NAPs）的水平与细菌群落组成、多样性和功能多样性密切相关，表明它们对细菌群落变化的驱动作用。总的来说，这项工作为理解人为活动在大河流域尺度上对细菌群落变化的贡献提供了依据。此外，研究结果为扩大生态评估提供了新的见解。
