Nomosatryo Sulung, Lipus Daniel, Bartholomäus Alexander, Henny Cynthia, Ridwansyah Iwan, Sujarta Puguh, Yang Sizhong, Wagner Dirk, Kallmeyer Jens
GFZ German Research Centre for Geosciences, Section Geomicrobiology, 14473, Potsdam, Germany.
Research Center for Limnology and Water Resources, National Research and Innovation Agency (BRIN), KST Soekarno, Jalan Jakarta-Bogor KM 46, Cibinong, Bogor 16911, Indonesia.
FEMS Microbiol Ecol. 2025 Jan 7;101(1). doi: 10.1093/femsec/fiae162.
Lake Sentani is a tropical lake in Indonesia, consisting of four interconnected sub-basins of different water depths. While previous work has highlighted the impact of catchment composition on biogeochemical processes in Lake Sentani, little is currently known about the microbiological characteristics across this unique ecosystem. With recent population growth in this historically rural area, the anthropogenic impact on Lake Sentani and hence its microbial life is also increasing. Therefore, we aimed to explore the influence of environmental and anthropogenic factors on the microbial diversity of Lake Sentani. Here, we present a detailed microbiological evaluation of Lake Sentani, analyzing 49 different sites across the lake, its tributary rivers and their river mouths to assess diversity and community structure using 16S rRNA gene sequencing. Our results reveal distinct communities in lake and river sediments, supporting the observed geochemical differences. Taxonomic assessment showed the potential impact of anthropogenic pressure along the northern, urbanized shore, as river and river mouth samples revealed high abundances of Bacteroidota, Firmicutes, and Cyanobacteria, which could be attributed to pollution and eutrophication. In contrast, lake sediment communities were dominated by Thermodesulfovibrionia, Methanomethylicia, Bathyarchaeia, and Thermoplasmata, suggesting sulfate reducing, thermophilic, acidophilic bacteria and methanogenic archaea to play an important role in tropical lake systems. This study provides novel insights into ecological functions of tropical lakes and contributes to the optimization of management strategies of Lake Sentani, ensuring its holistic preservation in the future.
塞塔尼湖是印度尼西亚的一个热带湖泊，由四个不同水深且相互连通的子流域组成。尽管此前的研究强调了集水区组成对塞塔尼湖生物地球化学过程的影响，但目前对于这个独特生态系统的微生物特征知之甚少。随着这个历史上的农村地区近期人口增长，人类活动对塞塔尼湖及其微生物群落的影响也在增加。因此，我们旨在探究环境和人为因素对塞塔尼湖微生物多样性的影响。在此，我们对塞塔尼湖进行了详细的微生物学评估，分析了该湖、其支流及其河口的49个不同地点，使用16S rRNA基因测序来评估多样性和群落结构。我们的结果揭示了湖泊和河流沉积物中的不同群落，这与观察到的地球化学差异相符。分类学评估显示，沿着北部城市化岸边存在人为压力的潜在影响，因为河流和河口样本显示拟杆菌门、厚壁菌门和蓝细菌的丰度很高，这可能归因于污染和富营养化。相比之下，湖泊沉积物群落主要由热脱硫弧菌纲、甲烷甲基菌纲、深海古菌纲和嗜热菌纲主导，这表明硫酸盐还原菌、嗜热菌、嗜酸菌和产甲烷古菌在热带湖泊系统中发挥着重要作用。这项研究为热带湖泊的生态功能提供了新的见解，并有助于优化塞塔尼湖的管理策略，确保其未来的整体保护。
