Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
PhD of physiology, Faculty of Nursing and Midwifery, Qom University of Medical Sciences, Qom, Iran.
Physiol Behav. 2022 Jan 1;243:113629. doi: 10.1016/j.physbeh.2021.113629. Epub 2021 Oct 29.
There is a sex difference in vulnerability to PTSD and in response to therapeutic interventions. Since relation between gonadal hormones and PTSD has been revealed, this study aimed to understand the severity of PTSD-induced impairments after ovarian hormone deficiency and the influence of exercise on PTSD accompanied by ovarian hormone deficiency. Female adult Wistar rats were subjected to ovariectomy, PTSD, or combination ovariectomy plus PTSD. Twenty days after ovariectomy, PTSD was induced by single prolonged stress (SPS) model. The exercise started 14 days after SPS and continued for 4 weeks. Thirty minutes moderate treadmill exercise was planned for 5 days per week. On day 65, after assessing rats using the elevated plus-maze (EPM) test, corticosterone, BDNF, and apoptotic markers were tested. p < 0.05 was considered as significant level. The results showed that ovariectomy worsened the effect of SPS on hippocampal BDNF and led to greater increase in serum corticosterone and hippocampal caspase 3 and BAX in SPS rats. Also, ovariectomy exacerbated anxiety-like behavior in SPS rats. Exercise improved the alterations of hippocampal BDNF, corticosterone, caspase 3, and BAX in SPS ovariectomized rats. However, exercise had no statistically significant effect on anxiety-like behavior in this group. According to the results, exercise is effective to attenuate SPS-induced impairments in molecular and cellular responses even when the condition becomes more complicated due to ovarian hormone deficiency. However, exercise alone cannot help to improve behavior impairments in PTSD combined with an ovarian hormone deficiency. Therefore, exercise could likely be considered as a complementary intervention to strengthen other treatments.
创伤后应激障碍（PTSD）易感性和治疗干预反应存在性别差异。由于已经揭示了性腺激素与 PTSD 之间的关系，因此本研究旨在了解卵巢激素缺乏引起的 PTSD 后损伤的严重程度，以及运动对伴有卵巢激素缺乏的 PTSD 的影响。将成年雌性 Wistar 大鼠进行卵巢切除术、PTSD 或卵巢切除术加 PTSD。卵巢切除术后 20 天，通过单次延长应激（SPS）模型诱导 PTSD。运动在 SPS 后 14 天开始，并持续 4 周。计划每周 5 天进行 30 分钟中等强度的跑步机运动。在第 65 天，通过高架十字迷宫（EPM）测试评估大鼠后，测试皮质酮、BDNF 和凋亡标志物。p<0.05 被认为是显著水平。结果表明，卵巢切除术加重了 SPS 对海马 BDNF 的影响，并导致 SPS 大鼠血清皮质酮和海马 caspase 3 和 BAX 增加。此外，卵巢切除术加剧了 SPS 大鼠的焦虑样行为。运动改善了 SPS 卵巢切除大鼠海马 BDNF、皮质酮、caspase 3 和 BAX 的改变。然而，运动对该组的焦虑样行为没有统计学上的显著影响。根据结果，运动可有效减轻 SPS 引起的分子和细胞反应改变，即使由于卵巢激素缺乏使情况变得更加复杂。然而，运动本身并不能帮助改善 PTSD 合并卵巢激素缺乏引起的行为损伤。因此，运动可能被认为是加强其他治疗的补充干预措施。
