The Gene is Necessary for the Proper Functioning of the Nogalamycin Biosynthesis Pathway.

Anthracycline drugs have multifunctional molecular structures, and small changes in the structure of the glycosyls around the chromophore affect their mechanism of action, pharmacokinetics, toxicity, anti-tumor activity, and many other significant parameters.  has a similar function to but inverse stereoselectivity. encoding amino transferase was substituted for for the purpose of obtaining nogalamycin analogues. We inactivated the gene encoding an aminotransferase responsible for the formation of nogalamine and introduced the gene encoding an aminotransferase responsible for the formation of daunosamine. We obtained the recombinant strain mLMX-3-100, in which the production of nogalamycin was disrupted. Interestingly, contrary to our predictions, no epi-nogalamycin was produced; nevertheless, the present study shows that the gene is necessary for the proper functioning of the nogalamycin biosynthesis pathway. These data may provide a reference for further illustration of nogalamycin biosynthesis and its modification by way of combinatorial biosynthesis.biosynthesis and its modification by way of combinatorial biosynthesis.