Ylihonko K, Hakala J, Kunnari T, Mäntsälä P
Department of Biochemistry and Food Chemistry, University of Turku, Finland.
Microbiology (Reading). 1996 Aug;142 ( Pt 8):1965-72. doi: 10.1099/13500872-142-8-1965.
A cluster of anthracycline biosynthetic genes isolated from Streptomyces nogalater was expressed in Streptomyces lividans and in Streptomyces galilaeus. A 12 kb DNA fragment cloned from this cluster in pIJ486 caused the production of a novel compound when introduced into S. lividans. The compound is derived from nogalonic acid methyl ester, an early intermediate in nogalamycin biosynthesis. Complementation with the cloned 12 kb fragment of S. galilaeus mutants blocked in aclacinomycin biosynthesis caused the production of hybrid anthracyclines. Cloning of the nogalamycin gene cluster should make possible a detailed study of the biosynthesis of this interesting antibiotic, as well as the production of novel anthracyclines of potential value as cytostatic drugs.
从诺加链霉菌中分离出的一组蒽环类生物合成基因在淡紫链霉菌和加利利链霉菌中表达。从该基因簇中克隆到pIJ486中的一个12 kb DNA片段导入淡紫链霉菌后可产生一种新化合物。该化合物衍生自诺加霉素生物合成早期中间体诺加洛酸甲酯。用在阿克拉霉素生物合成中受阻的加利利链霉菌突变体的克隆12 kb片段进行互补，可产生杂合蒽环类化合物。克隆诺加霉素基因簇将有可能详细研究这种有趣抗生素的生物合成，以及生产具有潜在细胞抑制药物价值的新型蒽环类化合物。
