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临床手术室中近红外一区(NIR-I)和短波红外(SWIR)荧光成像的注意事项。

Considerations for NIR-I and short-wave infrared (SWIR) fluorescence imaging within a clinical operating room.

作者信息

Byrd B K, Wirth D J, Paydarfar J A, Tafe L J, Samkoe K S, Paulsen K D, Davis S C

机构信息

Thayer School of Engineering at Dartmouth College, 14 Engineering Dr. Hanover, NH, 03755.

出版信息

Proc SPIE Int Soc Opt Eng. 2020 Feb;11222. doi: 10.1117/12.2543869. Epub 2020 Feb 24.

DOI:10.1117/12.2543869
PMID:34744248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8570453/
Abstract

Short-wave infrared (SWIR/NIR-II) fluorescence imaging has received increased attention for use in fluorescence-guided surgery (FGS) due to the potential for higher resolution imaging of subsurface structures and reduced autofluorescence compared to conventional NIR-I imaging. As with any fluorescence imaging modality introduced in the operating room, an appropriate accounting of contaminating background signal from other light sources in the operating room is an important step. Herein, we report the background signals in the SWIR and NIR-I emitted from commonly-used equipment in the OR, such as ambient and operating lights, LCD screens and surgical guidance systems. These results can guide implementation of protocols to reduce background signal.

摘要

由于与传统近红外I成像相比,短波红外(SWIR/NIR-II)荧光成像具有对皮下结构进行更高分辨率成像的潜力以及更低的自发荧光,因此在荧光引导手术(FGS)中的应用受到了越来越多的关注。与手术室中引入的任何荧光成像方式一样,对手术室中其他光源产生的污染背景信号进行适当的评估是重要的一步。在此,我们报告了手术室中常用设备发出的短波红外和近红外I背景信号,如环境光和手术灯、液晶显示屏及手术导航系统。这些结果可为减少背景信号的方案实施提供指导。

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本文引用的文献

1
First-in-human liver-tumour surgery guided by multispectral fluorescence imaging in the visible and near-infrared-I/II windows.基于可见-近红外 I/II 窗口多光谱荧光成像的首例人体肝脏肿瘤手术。
Nat Biomed Eng. 2020 Mar;4(3):259-271. doi: 10.1038/s41551-019-0494-0. Epub 2019 Dec 23.
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First experience imaging short-wave infrared fluorescence in a large animal: indocyanine green angiography of a pig brain.首例大动物短波近红外荧光成像:猪脑吲哚菁绿血管造影。
J Biomed Opt. 2019 Aug;24(8):1-4. doi: 10.1117/1.JBO.24.8.080501.
3
Characterizing short-wave infrared fluorescence of conventional near-infrared fluorophores.常规近红外荧光团的短波近红外荧光特性分析。
J Biomed Opt. 2019 Mar;24(3):1-5. doi: 10.1117/1.JBO.24.3.035004.
4
Absorption by water increases fluorescence image contrast of biological tissue in the shortwave infrared.水的吸收增加了生物组织在短波近红外光下的荧光图像对比度。
Proc Natl Acad Sci U S A. 2018 Sep 11;115(37):9080-9085. doi: 10.1073/pnas.1803210115. Epub 2018 Aug 27.
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Near-Infrared-II (NIR-II) Bioimaging Off-Peak NIR-I Fluorescence Emission.近红外二区(NIR-II)生物成像 非峰值近红外一区(NIR-I)荧光发射
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Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green.采用临床批准的近红外染料吲哚菁绿的短波近红外荧光成像。
Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):4465-4470. doi: 10.1073/pnas.1718917115. Epub 2018 Apr 6.
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Indocyanine green fluorescence in second near-infrared (NIR-II) window.第二近红外(NIR-II)窗口中的吲哚菁绿荧光。
PLoS One. 2017 Nov 9;12(11):e0187563. doi: 10.1371/journal.pone.0187563. eCollection 2017.
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Review of short-wave infrared spectroscopy and imaging methods for biological tissue characterization.用于生物组织表征的短波红外光谱和成像方法综述。
J Biomed Opt. 2015 Mar;20(3):030901. doi: 10.1117/1.JBO.20.3.030901.