Jain Mili, Rai Anushree, Kushwaha Rashmi, Yadav Geeta, Mishra Sanjay, Verma Shailendra Prasad, Singh Uma Shankar, Tripathi Anil Kumar, Kumar Ashutosh
Department of Pathology, King George's Medical University, Lucknow, 226003 India.
Department of Clinical Hematology, King George's Medical University, Lucknow, India.
Indian J Hematol Blood Transfus. 2021 Oct;37(4):563-568. doi: 10.1007/s12288-021-01397-x. Epub 2021 Jan 21.
T cells in B chronic lymphocytic leukaemia (CLL) have been reported to show qualitative and quantitative alterations, thereby regulating the antitumor immune response. We evaluated the absolute count, percentages of T cells and subsets and their association with disease parameters in Indian patients. 45 treatment naïve CLL cases and 10 healthy controls were evaluated for CD3 + Tcells, CD4 + T cells, CD8 + T cells percentage by flowcytometry. The absolute counts were obtained by multiplication with absolute lymphocyte counts obtained on cell counter. The clinical characteristics (age, sex, Rai stage, B symptoms, CD38 expression) were analysed for any association with alteration of these cells (percentages, absolute counts, ratio with monoclonal B cell count) and CD4: CD8 ratio. The mean absolute count of CD3 + T cell, CD4 + T cell and CD8 + T cells was significantly higher ( < 0.05) in CLL patients as compared to healthy controls. CD4:CD8 ratio was variable (normal, increased and decreased). The mean CD8 + T cells count was higher in advanced disease stage, and CD38 positive cases ( > 0.05). Younger CLL patients (< 55 years) had greater increase in CD8 + T cells ( > 0.05). Significant alterations in T cells and their subsets were observed in CLL. A trend towards advanced stage, CD38 expression, presentation an early age was seen with increase in CD8 + T cell counts.
据报道，B 细胞慢性淋巴细胞白血病（CLL）中的 T 细胞会出现定性和定量改变，从而调节抗肿瘤免疫反应。我们评估了印度患者 T 细胞及其亚群的绝对计数、百分比，以及它们与疾病参数的关联。通过流式细胞术对 45 例未经治疗的 CLL 病例和 10 例健康对照进行 CD3 + T 细胞、CD4 + T 细胞、CD8 + T 细胞百分比评估。绝对计数通过与血细胞计数器上获得的绝对淋巴细胞计数相乘得到。分析临床特征（年龄、性别、Rai 分期、B 症状、CD38 表达）与这些细胞的改变（百分比、绝对计数、与单克隆 B 细胞计数的比值）及 CD4:CD8 比值之间的任何关联。与健康对照相比，CLL 患者中 CD3 + T 细胞、CD4 + T 细胞和 CD8 + T 细胞的平均绝对计数显著更高（<0.05）。CD4:CD8 比值各不相同（正常、升高和降低）。晚期疾病阶段和 CD38 阳性病例中的 CD8 + T 细胞平均计数更高（>0.05）。年龄较轻的 CLL 患者（<55 岁）的 CD8 + T 细胞增加幅度更大（>0.05）。在 CLL 中观察到 T 细胞及其亚群有显著改变。随着 CD8 + T 细胞计数增加，可见疾病进展至晚期、CD38 表达、发病年龄较早的趋势。
