Rezaei-Chiyaneh Esmaeil, Mahdavikia Hassan, Hadi Hashem, Alipour Hadi, Kulak Muhittin, Caruso Gianluca, Siddique Kadambot H M
Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Iran.
Department of Medicinal Plants, Shahid Bakeri Higher Education Center of Miandoab, Urmia University, Urmia, Iran.
Physiol Mol Biol Plants. 2021 Oct;27(10):2201-2214. doi: 10.1007/s12298-021-01084-1. Epub 2021 Oct 13.
Cost-effective exogenous application of some antioxidant, viz. salicylic acid (SA) and ascorbic acid (AA), and essential micronutrient elements like Zn might alleviate the harmful impacts of drought stress. Here, we evaluated the interaction of foliar-sprayed SA (1 mM), AA (10 mM), and Zn (3 g L) and irrigation regime (normal irrigation, moderate water stress, and severe water stress) by assaying an array of agronomic, physiological, analytical and biochemical parameters of Moldavian balm ( L.). Accordingly, the SA and AA treatments reduced the harmful effects of moderate and severe drought stress. Well-watered plants applied with Zn had the highest biomass yield (4642.5 kg ha). Severe water stress decreased plant biomass, essential oil (EO) content, EO yield, relative water content, and chlorophyll content by 37.6%, 23.3%, 47.5%, 35.3%, and 53%, respectively, relative to normal irrigation. Plants treated with Zn under moderate drought stress had the highest EO content. Moderate and severe water stress increased enzymatic antioxidant (catalase, superoxide dismutase, and peroxidase) activities and total soluble sugars and proline contents. In terms of EO composition, SA-treated plants under moderate water stress contained the most geraniol (22.8%) and geranial (26.3%), while Zn-treated plants under severe water stress contained the most geranyl acetate (48.2%). This study demonstrated that foliar application of Zn and SA significantly improves EO productivity and quality in Moldavian balm under moderate water stress. The relevant findings were supported by heatmap clustering, revealing that irrigation regime had main effect on the essential oil compounds and biochemical and physiological parameters.
The online version contains supplementary material available at 10.1007/s12298-021-01084-1.
经济高效地外源施用某些抗氧化剂，即水杨酸（SA）和抗坏血酸（AA），以及必需的微量营养元素如锌，可能会减轻干旱胁迫的有害影响。在此，我们通过测定摩尔多瓦香脂（ ）的一系列农艺、生理、分析和生化参数，评估了叶面喷施SA（1 mM）、AA（10 mM）和锌（3 g/L）与灌溉制度（正常灌溉、中度水分胁迫和重度水分胁迫）之间的相互作用。相应地，SA和AA处理减轻了中度和重度干旱胁迫的有害影响。施锌的水分充足的植物生物量产量最高（4642.5 kg/公顷）。与正常灌溉相比，重度水分胁迫使植物生物量、精油（EO）含量、EO产量、相对含水量和叶绿素含量分别降低了37.6%、23.3%、47.5%、35.3%和53%。中度干旱胁迫下施锌处理的植物EO含量最高。中度和重度水分胁迫增加了酶促抗氧化剂（过氧化氢酶、超氧化物歧化酶和过氧化物酶）的活性以及总可溶性糖和脯氨酸的含量。就EO组成而言，中度水分胁迫下SA处理的植物中香叶醇（22.8%）和香叶醛（26.3%）含量最高，而重度水分胁迫下施锌处理的植物中乙酸香叶酯（48.2%）含量最高。本研究表明，在中度水分胁迫下，叶面喷施锌和SA可显著提高摩尔多瓦香脂的EO生产力和质量。热图聚类支持了相关发现，表明灌溉制度对精油化合物以及生化和生理参数有主要影响。
在线版本包含可在10.1007/s12298-021-01084-1获取的补充材料。
