Ren Xianyun, Wang Lei, Xu Yao, Wang Qiong, Lv Jianjian, Liu Ping, Li Jian
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Front Physiol. 2021 Oct 21;12:724693. doi: 10.3389/fphys.2021.724693. eCollection 2021.
, or the swimming crab, is tolerant of reduced salinity; however, the molecular mechanism of this tolerance is not clear. Cells can be damaged by hyperosmotic salinity. The protein p53, sometimes referred to as "the guardian of the genome," displays versatile and important functions under changing environmental conditions. Herein, the gene (designated as ) was cloned and studied. The full-length cDNA comprised 1,544bp, with a 1,314bp open reading frame, which encodes a putative polypeptide of 437 amino acids. Quantitative real-time reverse transcription PCR assays revealed ubiquitous expression of in all tissues examined, with the gills showing the highest expression level. Extensive apoptosis was detected under low salinity conditions using terminal deoxynucleotidyl transferase nick-end-labeling staining. Oxidative stress was induced under low salinity conditions, consequently leading to apoptosis. Low salinity stress caused significant upregulation of Ptp53 mRNA and protein levels in the gills. Moreover, compared with that in the control group, the mortality of -silenced crabs under low salinity stress was enhanced significantly. Taken together, our findings suggest that Ptp53, regulation of apoptosis and antioxidant defense, played important functions in the low salinity stress response of the swimming crab.
三疣梭子蟹，即游泳蟹，能够耐受盐度降低的环境；然而，这种耐受性的分子机制尚不清楚。高渗盐度会损害细胞。蛋白质p53有时被称为“基因组守护者”，在不断变化的环境条件下具有多种重要功能。在此，克隆并研究了三疣梭子蟹p53基因（命名为PtP53）。PtP53全长cDNA为1544bp，开放阅读框为1314bp，编码一个推定的437个氨基酸的多肽。实时定量逆转录PCR分析显示，PtP53在所检测的所有组织中均有广泛表达，其中鳃中的表达水平最高。使用末端脱氧核苷酸转移酶缺口末端标记染色法在低盐度条件下检测到广泛的细胞凋亡。低盐度条件下诱导了氧化应激，从而导致细胞凋亡。低盐度胁迫导致鳃中Ptp53 mRNA和蛋白质水平显著上调。此外，与对照组相比，PtP53基因沉默的螃蟹在低盐度胁迫下的死亡率显著提高。综上所述，我们的研究结果表明，Ptp53在调节细胞凋亡和抗氧化防御方面，在游泳蟹对低盐度胁迫的应答中发挥了重要作用。
