Cheng Chang-Hong, Ma Hai-Tao, Ma Hong-Ling, Liu Guang-Xin, Deng Yi-Qin, Feng Juan, Wang Li-Cang, Cheng Ying-Ying, Guo Zhi-Xun
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510300, China.
Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Aug;246:108976. doi: 10.1016/j.cbpc.2021.108976. Epub 2021 Jan 16.
The tumor suppressor protein p53 plays important roles in DNA repair, cell cycle and genetic stability. In the present study, a p53 gene in the mud crab (Scylla paramamosain) (designated as Sp-53) was identified and characterized. The open reading frame of Sp-53 was comprised a 1383 bp, which encoded a putative protein of 460 amino acids. Sp-53 is expressed in all examined tissues, with the highest expression in hepatopancreas and hemocytes. Vibrio parahaemolyticus infection induced oxidative stress, and led to DNA damage. The Sp-53 transcriptions in hepatopancreas were significantly up-regulated after V. parahaemolyticus infection. RNA interference (RNAi) experiment was used to understand the roles of Sp-53 in response to V. parahaemolyticus infection. Knocking down Sp-53 in vivo significantly reduced the expression of the Mn-SOD, Gpx3 and caspase 3 after V. parahaemolyticus infection. Moreover, the mortality of mud crabs and DNA damage in Sp-53-silenced mud crab challenged with V. parahaemolyticus were significantly higher than those in the control group. All these results suggested that Sp-53 played an important role in responses to V. parahaemolyticus infection through its participation in regulation of antioxidant defense, DNA repair and apoptosis.
肿瘤抑制蛋白p53在DNA修复、细胞周期和遗传稳定性方面发挥着重要作用。在本研究中，对拟穴青蟹（Scylla paramamosain）中的一个p53基因（命名为Sp-53）进行了鉴定和表征。Sp-53的开放阅读框由1383 bp组成，编码一个含有460个氨基酸的假定蛋白。Sp-53在所有检测的组织中均有表达，在肝胰腺和血细胞中的表达量最高。副溶血性弧菌感染诱导氧化应激，并导致DNA损伤。副溶血性弧菌感染后，肝胰腺中Sp-53的转录显著上调。采用RNA干扰（RNAi）实验来了解Sp-53在应对副溶血性弧菌感染中的作用。在体内敲低Sp-53后，副溶血性弧菌感染后Mn-SOD、Gpx3和caspase 3的表达显著降低。此外，用副溶血性弧菌攻击的Sp-53沉默拟穴青蟹的死亡率和DNA损伤显著高于对照组。所有这些结果表明，Sp-53通过参与抗氧化防御、DNA修复和细胞凋亡的调控，在应对副溶血性弧菌感染中发挥重要作用。
