Hetherington M L, Huntsman P R, Smith R G, Buchanan G R
Leuk Res. 1987;11(6):537-43. doi: 10.1016/0145-2126(87)90089-0.
Serial samples of peripheral blood were obtained from 75 children with acute lymphoblastic leukemia in remission. An immunofluorescence assay was used to quantitate TdT-containing (terminal deoxynucleotidyl transferase-containing) cells in the mononuclear leukocyte fraction of these specimens. Nine relapses in 8 patients were preceded by elevations (0.12-0.70%) in peripheral blood terminal deoxynucleotidyl transferase-containing cells noted 1-33 weeks prior to relapse. No such elevations were observed prior to 6 relapses in 4 patients. Peripheral blood terminal transferase deoxynucleotidyl-containing cells were elevated (0.12-0.61%) in 22 children who did not relapse over a 5-82 week period of observation. The sensitivity (67%) and specificity (68%) of this assay are inadequate to establish which patients have minimal residual leukemia.
从75例缓解期急性淋巴细胞白血病患儿中获取系列外周血样本。采用免疫荧光测定法对这些样本单核白细胞部分中含末端脱氧核苷酸转移酶（TdT）的细胞进行定量。8例患者出现9次复发，复发前1 - 33周外周血中含末端脱氧核苷酸转移酶的细胞升高（0.12 - 0.70%）。4例患者出现6次复发，复发前未观察到此类升高。在22例观察期为5 - 82周未复发的儿童中，外周血中含末端脱氧核苷酸转移酶的细胞升高（0.12 - 0.61%）。该测定法的敏感性（67%）和特异性（68%）不足以确定哪些患者存在微小残留白血病。
