Barr R D, Koekebakker M
Leuk Res. 1984;8(6):1051-5. doi: 10.1016/0145-2126(84)90060-2.
Serial samples of peripheral blood were obtained from 35 children with ALL over a period of 18 months. The mononuclear cells were examined for TdT by indirect immunofluorescence using an unpurified anti-calf thymus TdT as the primary antibody. This analysis failed to distinguish those children who were destined to relapse (n = 9) from those who remained in continuous complete remission. Rather, the exhibition of fluorescence was linked to the co-existence of infection, with a negative predictive value of 0.91. Putative 'TdT-positive' cells were concentrated in the T-lymphocyte fraction and the very process of E-rosette formation seemed to contribute to this phenomenon. It appears as if the anti-TdT reagent recognizes not only TdT but also a variety of antigens which are expressed on or in immature and activated lymphocytes.
在18个月的时间里，从35名急性淋巴细胞白血病患儿身上采集了外周血系列样本。使用未纯化的抗小牛胸腺末端脱氧核苷酸转移酶（TdT）作为一抗，通过间接免疫荧光法检测单核细胞中的TdT。该分析未能区分那些注定会复发的儿童（n = 9）和那些持续完全缓解的儿童。相反，荧光的出现与感染的并存有关，阴性预测值为0.91。假定的“TdT阳性”细胞集中在T淋巴细胞部分，而E玫瑰花结形成过程似乎促成了这一现象。似乎抗TdT试剂不仅识别TdT，还识别在未成熟和活化淋巴细胞上或其中表达的多种抗原。
