Laboratory of Aquaculture & Artemia Reference Center, Department of Animal Science and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Front Immunol. 2021 Oct 20;12:693932. doi: 10.3389/fimmu.2021.693932. eCollection 2021.
The halophilic aquatic bacterium is an important aquatic pathogen, capable of causing vibriosis in shrimp and fish resulting in significant economic losses. In a previous work, essential oils (EOs) extracts from , , and were found to inhibit the growth of . This study aimed to determine EOs' potential protective effect towards gnotobiotic brine shrimp , challenged with . The study showed that brine shrimp larvae supplemented with EOs of (0.0008%) and (0.002%) displayed significantly increased survival against . The results indicated that supplementation of these EOs increased the expression of immune-related genes (either in the presence or absence of the pathogen), probably contributing to enhanced protection. Furthermore, studies indicated that some EOs modulated the expression of virulence factors including swimming motility, biofilm formation, and gelatinase and lipase activity, while flow cytometry data and regrowth assay indicated that these EOs do not exhibit antimicrobial activity as grew at the tested concentrations [ (0.0008%) and (0.002%)]. Our findings suggest that EOs extracted from and , can modulate virulence factor production and immunological responses and might hence become part of an intervention strategy to control vibriosis in a fish or shrimp aquaculture setting, a hypothesis that needs to be validated in the future.
海洋嗜盐菌是一种重要的水生病原体，能够引起虾和鱼类的弧菌病，导致重大的经济损失。在之前的研究中，发现 、 、 的精油提取物能够抑制 的生长。本研究旨在确定海洋嗜盐菌精油对无菌卤虫的潜在保护作用，卤虫受到 的挑战。研究表明，用浓度为 0.0008%和 0.002%的精油处理的卤虫幼虫的存活率显著提高。结果表明，这些精油的补充增加了免疫相关基因的表达（无论是否存在病原体），这可能有助于增强保护作用。此外，研究表明，一些精油调节了包括游泳运动性、生物膜形成、明胶酶和脂肪酶活性在内的毒力因子的表达，而流式细胞术数据和再生试验表明，这些精油在测试浓度下（0.0008%和 0.002%）不具有抗菌活性。我们的研究结果表明，从 和 中提取的精油可以调节毒力因子的产生和免疫反应，因此可能成为控制鱼类或虾类水产养殖中弧菌病的干预策略的一部分，这一假设需要在未来得到验证。
