Liu Wenzhe, Yin Tongyang, Zhao Yufei, Wang Xueqin, Wang Kailu, Shen Yingying, Ding Yanfeng, Tang She
College of Agronomy, Nanjing Agricultural University, Nanjing, China.
Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.
Front Plant Sci. 2021 Oct 21;12:746180. doi: 10.3389/fpls.2021.746180. eCollection 2021.
With the intensification of global warming, rice production is facing new challenges. Field evidence indicates that elevated temperature during rice grain-filling leads to the further deterioration of grain quality. In order to clarify the potential regulatory mechanism of elevated temperature on the formation of rice quality, the DIA mass spectrometry method under the background of field warming was conducted to investigate the regulatory effects of high temperature on grain development and material accumulation pathways. The results showed that a total of 840 differentially expressed proteins were identified during the grain-filling process under elevated temperature. These differentially expressed proteins participated in carbon metabolism, amino acid biosynthesis, signal transduction, protein synthesis, and alternately affected the material accumulation of rice grains. The significant up-regulation of PPROL 14E, PSB28, granule-bound starch synthase I, and the significant down-regulation of 26.7 kDa heat shock protein would lead to the component difference in grain starch and storage proteins, and that could be responsible for the degradation of rice quality under elevated temperature. Results suggested that proteins specifically expressed under elevated temperature could be the key candidates for elucidating the potential regulatory mechanism of warming on rice development and quality formation. In-depth study on the metabolism of storage compounds would be contributed in further proposing high-quality cultivation control measures suitable for climate warming.
随着全球变暖的加剧，水稻生产面临新的挑战。田间证据表明，水稻灌浆期温度升高会导致稻米品质进一步恶化。为阐明温度升高对水稻品质形成的潜在调控机制，在田间增温背景下采用DIA质谱法，研究高温对籽粒发育及物质积累途径的调控作用。结果表明，在高温条件下的灌浆过程中，共鉴定出840个差异表达蛋白。这些差异表达蛋白参与碳代谢、氨基酸生物合成、信号转导、蛋白质合成，并交替影响水稻籽粒的物质积累。PPROL 14E、PSB28、颗粒结合型淀粉合成酶I的显著上调，以及26.7 kDa热休克蛋白的显著下调，会导致籽粒淀粉和贮藏蛋白的成分差异，这可能是高温下水稻品质下降的原因。结果表明，高温特异表达的蛋白可能是阐明增温对水稻发育和品质形成潜在调控机制的关键候选蛋白。深入研究贮藏化合物的代谢，将有助于进一步提出适合气候变暖的优质栽培调控措施。
