Alhumaydhi Fahad A, Aljohani Abdullah S M, Elsharkawy Eman R
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.
Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia.
Evid Based Complement Alternat Med. 2021 Oct 28;2021:4718854. doi: 10.1155/2021/4718854. eCollection 2021.
Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of in streptozotocin-induced diabetic rats were investigated . A single intraperitoneal injection of 160 mg/kg bodyweights of streptozotocin was used to cause diabetes. Blood glucose levels were tested with an AccuCheck Advantage II glucometer and blood glucose test strips. After diabetes was confirmed, animals were orally treated with the extract, metformin, and insulin according to the experimental design. After extract therapy, histological alterations in the pancreas of diabetic rats were investigated. When compared to a control group, daily oral administration of extract (300 mg/kg body weight) plus metformin (100 mg/kg) had a positive effect on blood glucose levels as well as showed an increased number of white blood cells (WBCs) and red blood cells (RBCs). The treatment with the extract for two weeks showed a positive impact on pancreatic histopathological changes in the groups with the diabetic rats. Phenolic fraction of the methanol extract was screened by the liquid chromatography-mass spectroscopy (LC-MS) method, which unveiled the existence of flavonoid compounds and phenolics as kaempferol, rutin, isorhamnetin-3-O-rutinoside, caffeic acid, and 4-hydroxybenzoic acid 4-O-glucoside. The results confirmed the use of the plant as an antidiabetic agent; the research recommended further studies on the plant to use the plant as an antidiabetic drug, where the plant extract also showed improvement in blood parameters.
草药植物是降血糖抗糖尿病药物的新来源；对链脲佐菌素诱导的糖尿病大鼠的甲醇提取物进行了血液学和组织病理学研究。单次腹腔注射160mg/kg体重的链脲佐菌素以诱发糖尿病。使用AccuCheck Advantage II血糖仪和血糖测试条检测血糖水平。确认糖尿病后，根据实验设计对动物进行提取物、二甲双胍和胰岛素的口服治疗。提取物治疗后，研究糖尿病大鼠胰腺的组织学改变。与对照组相比，每日口服提取物（300mg/kg体重）加二甲双胍（100mg/kg）对血糖水平有积极影响，同时白细胞（WBC）和红细胞（RBC）数量增加。提取物治疗两周对糖尿病大鼠组的胰腺组织病理学变化有积极影响。通过液相色谱 - 质谱（LC - MS）方法对甲醇提取物的酚类部分进行筛选，结果显示存在黄酮类化合物和酚类物质，如槲皮素、芦丁、异鼠李素 - 3 - O - 芸香糖苷、咖啡酸和4 - 羟基苯甲酸4 - O - 葡萄糖苷。结果证实该植物可作为抗糖尿病药物；研究建议对该植物进行进一步研究，以便将其用作抗糖尿病药物，该植物提取物还显示出血液参数的改善。
