Zhang Feng, Xu Yanyun, Shen Liye, Huang Junjie, Xu Songtao, Li Jiaying, Sun Zhichao, He Jiangmin, Chen Minli, Pan Yongming
The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Institute of Comparative Medicine, Experimental Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Evid Based Complement Alternat Med. 2021 Oct 28;2021:9253281. doi: 10.1155/2021/9253281. eCollection 2021.
Based on accumulating evidence, Alzheimer's disease (AD) is related to hypercholesterolemia, gut microbiota, and host metabolites. GuanXinNing Tablet (GXN) is an oral compound preparation composed of two Chinese herbs, Bge. and Hort., both of which exert neuroprotective effects. Nevertheless, the effect of GXN on AD is unknown. In the present study, we investigated whether GXN alters cholesterol, amyloid-beta (A), gut microbiota, serum metabolites, oxidative stress, neuronal metabolism activities, and apoptosis in an AD model rabbit fed a 2% cholesterol diet. Our results suggested that the GXN treatment significantly reduced cholesterol levels and A deposition and improved memory and behaviors in AD rabbits. The 16S rRNA analysis showed that GXN ameliorated the changes in the gut microbiota, decreased the ratio, and improved the abundances of and . H-NMR metabolomics found that GXN regulated 12 different serum metabolites, such as low-density lipoprotein (LDL), trimethylamine N-oxide (TMAO), and glutamate (Glu). In addition, the H-MRS examination showed that GXN remarkably increased N-acetyl aspartate (NAA) and Glu levels while reducing myo-inositol (mI) and choline (Cho) levels in AD rabbits, consequently enhancing neuronal metabolism activities. Furthermore, GXN significantly inhibited oxidative stress and neuronal apoptosis. Taken together, these results indicate that GXN attenuates AD via improving gut microbiota, host metabolites, and neuronal apoptosis.
基于越来越多的证据，阿尔茨海默病（AD）与高胆固醇血症、肠道微生物群和宿主代谢产物有关。冠心宁片（GXN）是一种由两种中药，即薤白（Bge.）和山楂（Hort.）组成的口服复方制剂，二者均具有神经保护作用。然而，GXN对AD的影响尚不清楚。在本研究中，我们调查了GXN是否会改变喂食2%胆固醇饮食的AD模型兔的胆固醇、β淀粉样蛋白（A）、肠道微生物群、血清代谢产物、氧化应激、神经元代谢活性和细胞凋亡。我们的结果表明，GXN治疗显著降低了AD兔的胆固醇水平和A沉积，并改善了其记忆和行为。16S rRNA分析表明，GXN改善了肠道微生物群的变化，降低了[具体比例]，并提高了[具体菌种1]和[具体菌种2]的丰度。氢核磁共振代谢组学发现，GXN调节了12种不同的血清代谢产物，如低密度脂蛋白（LDL）、氧化三甲胺（TMAO）和谷氨酸（Glu）。此外，氢磁共振波谱检查表明，GXN显著提高了AD兔脑中N-乙酰天门冬氨酸（NAA）和Glu水平，同时降低了肌醇（mI）和胆碱（Cho）水平，从而增强了神经元代谢活性。此外，GXN显著抑制了氧化应激和神经元凋亡。综上所述，这些结果表明，GXN通过改善肠道微生物群、宿主代谢产物和神经元凋亡来减轻AD。
