Juvekar A S, Chitnis M P, Advani S H
Neoplasma. 1987;34(2):199-204.
Adriamycin and mitoxantrone are known antitumor agents. The use of these agents is limited by their toxicity to normal body tissue. This paper shows that it is possible to achieve greater log cell-kill by using these drugs in combination with hyperthermia and diazepam. Experiments were carried out on 22 human chronic myeloid leukemia samples. 10 micrograms/ml adriamycin and 1 microgram/ml mitoxantrone were used in combination with hyperthermia 42 degrees C, for 3 h and 1 h respectively, with and without diazepam (1 microgram/ml). Inhibition of incorporation of radiolabeled nucleic acid precursor (3H-thymidine) in treated cells as compared to the untreated cells was used as a measure of cytotoxicity. The statistical evaluation of the data shows that the enhancement of drug cytotoxicity due to hyperthermia and diazepam is highly significant (p less than 0.001) in case of both the drugs.
阿霉素和米托蒽醌是已知的抗肿瘤药物。这些药物的使用受到其对正常身体组织毒性的限制。本文表明，将这些药物与热疗和地西泮联合使用有可能实现更大程度的对数细胞杀伤。对22份人类慢性髓性白血病样本进行了实验。分别使用10微克/毫升阿霉素和1微克/毫升米托蒽醌，与42摄氏度的热疗联合使用，分别持续3小时和1小时，同时使用和不使用地西泮（1微克/毫升）。与未处理细胞相比，处理后细胞中放射性标记核酸前体（3H-胸腺嘧啶核苷）掺入的抑制被用作细胞毒性的一种度量。数据的统计评估表明，在两种药物的情况下，热疗和地西泮导致的药物细胞毒性增强都非常显著（p小于0.001）。
