miR-19a-3p 通过靶向 THBS1 促进膀胱尿路上皮癌的肿瘤相关行为。
miR-19a-3p Promotes Tumor-Relevant Behaviors in Bladder Urothelial Carcinoma via Targeting THBS1.
机构信息
Department of Urology, Shaoxing People's Hospital (Shaoxing Hospital), Zhejiang University School of Medicine, Shaoxing City, Zhejiang Province 312000, China.
出版信息
Comput Math Methods Med. 2021 Oct 28;2021:2710231. doi: 10.1155/2021/2710231. eCollection 2021.
OBJECTIVE
miR-19a-3p is widely increased in several cancers and can be used as an oncogenic factor in these cancers. However, the molecular mechanism of miR-19a-3p in bladder urothelial carcinoma (BLCA) is still open. So, the study was aimed at exploring the mechanism of miR-19a-3p in BLCA cells.
METHODS
Bioinformatics analysis was employed to find the differential miRNAs and mRNAs, and the target miRNA and mRNA were determined. Real-time quantitative PCR was used to evaluate miR-19a-3p and THBS1 levels in human urethral epithelial cells and BLCA cells. Western blot was carried out to assay protein expression of THBS1 in human urethral epithelial cells and BLCA cells. Behaviors of BLCA cells were detected through cellular functional assays. Dual-luciferase gene assay was conducted to validate the binding of miR-19a-3p and THBS1.
RESULTS
miR-19a-3p was increased in BLCA cells, while THBS1 was less expressed in BLCA cells. The miR-19a-3p functions as an oncogene in BLCA. THBS1 was a target of miR-19a-3p, and it could reverse the promotion of miR-19a-3p on cell malignant behaviors in BLCA.
CONCLUSION
miR-19a-3p facilitates cell progression in BLCA via binding THBS1, which may be an underlying therapeutic target for BLCA treatment.
目的
miR-19a-3p 在多种癌症中广泛增加，可作为这些癌症中的致癌因子。然而，miR-19a-3p 在膀胱尿路上皮癌 (BLCA) 中的分子机制尚不清楚。因此，本研究旨在探讨 miR-19a-3p 在 BLCA 细胞中的作用机制。
方法
采用生物信息学分析寻找差异表达的 miRNA 和 mRNAs，确定靶 miRNA 和 mRNA。实时定量 PCR 评估人尿道上皮细胞和 BLCA 细胞中 miR-19a-3p 和 THBS1 的水平。Western blot 检测人尿道上皮细胞和 BLCA 细胞中 THBS1 的蛋白表达。通过细胞功能测定检测 BLCA 细胞的行为。双荧光素酶基因检测验证 miR-19a-3p 和 THBS1 的结合。
结果
miR-19a-3p 在 BLCA 细胞中升高，而 THBS1 在 BLCA 细胞中表达降低。miR-19a-3p 在 BLCA 中作为癌基因发挥作用。THBS1 是 miR-19a-3p 的靶标，可逆转 miR-19a-3p 对 BLCA 细胞恶性行为的促进作用。
结论
miR-19a-3p 通过结合 THBS1 促进 BLCA 细胞进展，可能是 BLCA 治疗的潜在治疗靶点。
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