Souza Rosiléia Silva, Machado William Morais, da França Caline Santana, Mugabe Lopes César, Pinheiro Emmanuel Emydio Gomes, Carneiro Isabella de Matos Brandão, Rocha Laiara Fernandes, Barbosa Larissa Pires
Programa de Pós-graduação em Ciências Animal, Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brasil.
Anim Reprod. 2021 Oct 29;18(3):e20210027. doi: 10.1590/1984-3143-AR2021-0027. eCollection 2021.
The effects of docosahexaenoic acid (DHA) in the diluent for cryopreservation of goat semen on seminal quality and the optimal levels to be used were evaluated. After collection, semen was pooled and physically evaluated, then divided into four aliquots with different DHA levels in the diluent: 0, 10, 20, and 30 ng mL. The semen was cryopreserved in a TK 3000® freezing machine and then thawed for assessment at 37 °C. Sperm motility and vigor, membrane integrity, acrosomal integrity, mitochondrial activity, and sperm chromatin compaction were evaluated after thawing. A completely randomized design was used. For normally distributed variables, ANOVA and regression analysis were used to test for differences between treatments, and for non-parametric data, the Kruskal Wallis test was used at the 5% significance level. There were no differences among groups in terms of membrane integrity, acrosomal integrity, or chromatin compaction. There was a decrease in class I mitochondrial activity with increasing DHA level (P<0.05), but no differences in classes II, III, and IV (P>0.05). The inclusion of 10 to 30 ng mL of DHA in the diluent did not result in improvements in seminal quality parameters after thawing, with some impairment observed in the mitochondrial activity of the sperm cells.
评估了二十二碳六烯酸（DHA）在山羊精液冷冻保存稀释剂中对精液质量的影响以及最佳使用水平。采集后，将精液汇集并进行物理评估，然后分为四份，稀释剂中DHA水平不同：0、10、20和30 ng/mL。精液在TK 3000®冷冻机中冷冻保存，然后在37°C解冻进行评估。解冻后评估精子活力、活力、膜完整性、顶体完整性、线粒体活性和精子染色质浓缩情况。采用完全随机设计。对于正态分布变量，使用方差分析和回归分析来检验处理之间的差异，对于非参数数据，在5%显著性水平下使用Kruskal Wallis检验。各组在膜完整性、顶体完整性或染色质浓缩方面没有差异。随着DHA水平的增加，I类线粒体活性降低（P<0.05），但II、III和IV类没有差异（P>0.05）。在稀释剂中加入10至30 ng/mL的DHA并不能改善解冻后的精液质量参数，且观察到精子细胞的线粒体活性有一定损害。
