Ohba Shinsuke
Department of Cell Biology, Institute of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan.
Jpn Dent Sci Rev. 2021 Nov;57:217-223. doi: 10.1016/j.jdsr.2021.10.001. Epub 2021 Oct 25.
The mammalian skeleton develops through two distinct modes of ossification: intramembranous ossification and endochondral ossification. During the process of skeletal development, SRY-box containing gene 9 (Sox9), runt-related transcription factor 2 (Runx2), and Sp7 work as master transcription factors (TFs) or transcriptional regulators, underlying cell fate specification of the two distinct populations: bone-forming osteoblasts and cartilage-forming chondrocytes. In the past two decades, core transcriptional circuits underlying skeletal development have been identified mainly through mouse genetics and biochemical approaches. Recently emerging next-generation sequencer (NGS)-based studies have provided genome-scale views on the gene regulatory landscape programmed by the master TFs/transcriptional regulators. With particular focus on Sox9, Runx2, and Sp7, this review aims to discuss the gene regulatory landscape in skeletal development, which has been identified by genome-scale data, and provide future perspectives in this field.
膜内骨化和软骨内骨化。在骨骼发育过程中，含SRY盒基因9（Sox9）、 runt相关转录因子2（Runx2）和Sp7作为主要转录因子（TFs）或转录调节因子，决定了两种不同细胞群体的命运：形成骨的成骨细胞和形成软骨的软骨细胞。在过去二十年中，骨骼发育的核心转录回路主要通过小鼠遗传学和生化方法得以确定。最近基于新一代测序仪（NGS）的研究提供了由主要TFs/转录调节因子编程的基因调控全景图。本文特别聚焦于Sox9、Runx2和Sp7，旨在讨论通过基因组规模数据确定的骨骼发育中的基因调控全景图，并提供该领域的未来展望。
