Assal Nadia, Rennie Bryan, Walrond Lisa, Cyr Terry, Rohonczy Liz, Lin Min
Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON, Canada.
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
Biochem Biophys Rep. 2021 Oct 23;28:101154. doi: 10.1016/j.bbrep.2021.101154. eCollection 2021 Dec.
This study aimed to identify proteins secreted by into culture medium at different stages of bacterial growth. A field strain of was grown in Middlebrook 7H9 media and culture supernatant was collected at three-time points representing three different phases of growth (early exponential, late exponential, and stationary phases). Supernatants were double filtered, digested by trypsin and analyzed by LC-MS/MS. The study found 15, 21, and 16 proteins in early, mid and late growth phases, respectively. In total, 22 proteins were identified, 18 of which were reported or predicted to have a cell wall or extracellular localization. To our knowledge, this is the first study to identify proteins secreted into the culture medium by a field strain of in three different stages of growth. The dataset generated here provides candidate proteins with the potential for the development of serological diagnostic reagents or vaccine for bovine tuberculosis. Data are available via ProteomeXchange with identifier PXD017817.
本研究旨在鉴定在细菌生长不同阶段分泌到培养基中的蛋白质。一株野外菌株在Middlebrook 7H9培养基中培养，并在代表三个不同生长阶段（指数前期、指数后期和稳定期）的三个时间点收集培养上清液。上清液经过双重过滤，用胰蛋白酶消化，并通过液相色谱-串联质谱进行分析。该研究分别在生长早期、中期和后期发现了15种、21种和16种蛋白质。总共鉴定出22种蛋白质，其中18种据报道或预测具有细胞壁或细胞外定位。据我们所知，这是第一项在三个不同生长阶段鉴定野外菌株分泌到培养基中的蛋白质的研究。此处生成的数据集提供了具有开发牛结核病血清学诊断试剂或疫苗潜力的候选蛋白质。数据可通过ProteomeXchange获得，标识符为PXD017817。
