School of Geography, Earth and Environmental Sciences, University of Birmingham.
UK Centre for Ecology and Hydrology; Natural England.
J Vis Exp. 2021 Oct 25(176). doi: 10.3791/61764.
The physicochemical characterization of nanomaterials (NMs) is often an analytical challenge, due to their small size (at least one dimension in the nanoscale, i.e. 1-100 nm), dynamic nature, and diverse properties. At the same time, reliable and repeatable characterization is paramount to ensure safety and quality in the manufacturing of NM-bearing products. There are several methods available to monitor and achieve reliable measurement of nanoscale-related properties, one example of which is Ultraviolet-Visible Spectroscopy (UV-Vis). This is a well-established, simple, and inexpensive technique that provides non-invasive and fast real-time screening evaluation of NM size, concentration, and aggregation state. Such features make UV-Vis an ideal methodology to assess the proficiency testing schemes (PTS) of a validated standard operating procedure (SOP) intended to evaluate the performance and reproducibility of a characterization method. In this paper, the PTS of six partner laboratories from the H2020 project ACEnano were assessed through an interlaboratory comparison (ILC). Standard gold (Au) colloid suspensions of different sizes (ranging 5-100 nm) were characterized by UV-Vis at the different institutions to develop an implementable and robust protocol for NM size characterization.
纳米材料（NMs）的物理化学特性通常是一个分析挑战，因为它们的尺寸小（至少一个维度在纳米范围内，即 1-100nm）、动态性质和多样的性质。同时，可靠和可重复的特性描述对于确保含有 NM 的产品的制造安全和质量至关重要。有几种方法可用于监测和实现对纳米相关特性的可靠测量，其中一种方法是紫外可见光谱（UV-Vis）。这是一种成熟、简单且廉价的技术，可提供非侵入性和快速实时的 NM 尺寸、浓度和聚集状态的筛选评估。这些特点使 UV-Vis 成为评估经过验证的标准操作程序（SOP）的能力验证计划（PTS）的理想方法，旨在评估一种特性描述方法的性能和重现性。在本文中，通过实验室间比较（ILC）评估了 H2020 项目 ACEnano 的六个合作实验室的 PTS。使用 UV-Vis 在不同的机构中对不同尺寸（5-100nm 范围内）的标准金（Au）胶体悬浮液进行了特性描述，以开发出一种可实施且稳健的 NM 尺寸特性描述协议。
