Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands.
School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Molecules. 2021 Sep 1;26(17):5315. doi: 10.3390/molecules26175315.
ACEnano is an EU-funded project which aims at developing, optimising and validating methods for the detection and characterisation of nanomaterials (NMs) in increasingly complex matrices to improve confidence in the results and support their use in regulation. Within this project, several interlaboratory comparisons (ILCs) for the determination of particle size and concentration have been organised to benchmark existing analytical methods. In this paper the results of a number of these ILCs for the characterisation of NMs are presented and discussed. The results of the analyses of pristine well-defined particles such as 60 nm Au NMs in a simple aqueous suspension showed that laboratories are well capable of determining the sizes of these particles. The analysis of particles in complex matrices or formulations such as consumer products resulted in larger variations in particle sizes within technologies and clear differences in capability between techniques. Sunscreen lotion sample analysis by laboratories using spICP-MS and TEM/SEM identified and confirmed the TiO particles as being nanoscale and compliant with the EU definition of an NM for regulatory purposes. In a toothpaste sample orthogonal results by PTA, spICP-MS and TEM/SEM agreed and stated the TiO particles as not fitting the EU definition of an NM. In general, from the results of these ILCs we conclude that laboratories are well capable of determining particle sizes of NM, even in fairly complex formulations.
ACEnano 是一个由欧盟资助的项目，旨在开发、优化和验证用于检测和表征日益复杂基质中纳米材料 (NMs) 的方法，以提高结果的可信度并支持其在法规中的应用。在该项目中，组织了多次用于确定粒径和浓度的实验室间比较 (ILC)，以对现有分析方法进行基准测试。本文介绍并讨论了这些 ILC 中用于表征 NMs 的一些结果。对简单水悬浮液中 60nm Au NMs 等原始定义良好的颗粒进行的分析结果表明，实验室能够很好地确定这些颗粒的大小。对复杂基质或配方（如消费品）中的颗粒进行分析，导致不同技术之间的粒径变化较大，并且技术之间的能力存在明显差异。使用 spICP-MS 和 TEM/SEM 的实验室对防晒霜样品进行分析，识别并确认 TiO 颗粒为纳米级，符合欧盟对 NM 的监管定义。在牙膏样品中，PTA、spICP-MS 和 TEM/SEM 的正交结果一致，并指出 TiO 颗粒不符合欧盟对 NM 的定义。总的来说，从这些 ILC 的结果来看，我们得出结论，实验室能够很好地确定 NM 的粒径，即使在相当复杂的配方中也是如此。
