China National Tobacco Quality Supervision and Test Center, Zhengzhou 450001, China.
Key Laboratory of Tobacco Biological Effects, Zhengzhou 450001, China.
Chem Res Toxicol. 2021 Dec 20;34(12):2460-2470. doi: 10.1021/acs.chemrestox.1c00224. Epub 2021 Nov 8.
Harmful and potentially harmful constituents (HPHCs) in tobacco smoke are thought to be responsible for the increased health risks. Tobacco heating products (THPs) heat tobacco instead of burning it to achieve significantly fewer toxicants than conventional cigarettes. To assess the toxicity of THP aerosols, it is often desirable to extract the main constituents using a solvent method. In this study, we developed a high-speed centrifugal method for extracting the total particulate matter (TPM) from THPs to quantitatively compare the toxicity of different THPs and conventional cigarettes. Its TPM extraction efficiency exceeded 85%, and the primary aerosol components and typical HPHCs were comparable to those of the solvent method. The TPMs extracted from five THPs were subjected to 14 in vitro toxicology assessments, and the results were compared with those of a 3R4F reference cigarette. Physical separation can improve biases from solvent selectivity and potential interactions between solvent and aerosol constituents. By eliminating solvent influence, the extraction method could achieve high-dose exposures, enabling the toxicity comparison of different THPs. The relative toxicity of the THPs differed under different dosage units, including the TPM concentration, nicotine equivalent, and puff number.
烟草烟雾中的有害和潜在有害成分（HPHCs）被认为是导致健康风险增加的原因。加热不燃烧烟草制品（THPs）通过加热而非燃烧来实现，其产生的有毒物质比传统香烟显著减少。为了评估 THP 气溶胶的毒性，通常希望使用溶剂法提取主要成分。在这项研究中，我们开发了一种高速离心法从 THP 中提取总颗粒物（TPM），以定量比较不同 THP 和传统香烟的毒性。其 TPM 提取效率超过 85%，且主要气溶胶成分和典型 HPHCs 与溶剂法相当。从 5 种 THP 中提取的 TPM 进行了 14 项体外毒理学评估，并将结果与 3R4F 参考香烟进行了比较。物理分离可以减少溶剂选择性和溶剂与气溶胶成分之间潜在相互作用的偏差。通过消除溶剂的影响，提取方法可以实现高剂量暴露，从而对不同 THP 的毒性进行比较。不同的 THP 在不同的剂量单位下（包括 TPM 浓度、尼古丁当量和吸烟口数）表现出不同的相对毒性。
