Canli Esin G, Celenk Alper, Canli Mustafa
Department of Biology, Faculty of Sciences and Arts, University of Nigde Omer Halisdemir, Nigde, Turkey.
Department of Histology and Embryology, Faculty of Veterinary of Ceyhan, University of Cukurova, Adana, Turkey.
Bull Environ Contam Toxicol. 2022 Apr;108(4):702-707. doi: 10.1007/s00128-021-03410-5. Epub 2021 Nov 8.
Freshwater mussels are used as an effective bioindicator of metal pollution. There is no data on the accumulation of any metal-oxide nanoparticles (NPs) in tissues of Unio tigridis. Thus, this study was undertaken to investigate accumulation of AlO, CuO, and TiO NPs following exposure to different concentrations (0, 1, 3, and 9 mg/L) of NPs for 14 days. Metal concentrations in tissues were determined by ICP-MS, while NP presence was demonstrated by transmission electron microscope (TEM) images. During the experiments, mussels were fed with cultured algae (Chlorella vulgaris). TEM images demonstrated the presence of NPs in digestive gland and muscle. TEM images also suggested that NPs were taken via the lysosomes or endosomes. Highest mean concentrations (µg/g d.w.) of aluminium (76.51), copper (111.63) and titanium (113.83) occurred in the gills and followed by the digestive glands and muscles. Algae consumption of mussels did not significantly differ among controls and NP-exposed groups.
淡水贻贝被用作金属污染的有效生物指标。目前尚无关于乌苏里珠蚌组织中任何金属氧化物纳米颗粒（NPs）积累的数据。因此，本研究旨在调查暴露于不同浓度（0、1、3和9毫克/升）的纳米颗粒14天后，氧化铝、氧化铜和二氧化钛纳米颗粒的积累情况。通过电感耦合等离子体质谱法（ICP-MS）测定组织中的金属浓度，同时通过透射电子显微镜（TEM）图像证明纳米颗粒的存在。在实验过程中，贻贝以培养的藻类（普通小球藻）为食。透射电子显微镜图像证明消化腺和肌肉中存在纳米颗粒。透射电子显微镜图像还表明，纳米颗粒是通过溶酶体或内吞体摄取的。鳃中铝（76.51）、铜（111.63）和钛（113.83）的平均浓度最高（微克/克干重），其次是消化腺和肌肉。对照组和纳米颗粒暴露组之间贻贝的藻类消耗量没有显著差异。
