Canli Esin G, Baykose Ahmet, Uslu Leyla Hizarci, Canli Mustafa
University of Cukurova, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Adana, Turkey.
University of Cukurova, Faculty of Sciences and Arts, Department of Biology, Adana, Turkey.
Environ Toxicol Pharmacol. 2023 Mar;98:104077. doi: 10.1016/j.etap.2023.104077. Epub 2023 Feb 4.
In this study, responses of various biomarkers in the digestive gland and foot muscle of freshwater mussels (Unio tigridis) were investigated following exposure to AlO, CuO and TiO nanoparticles (NPs) for 14 days at different concentrations (0, 1, 3 and 9 mg NP/L). Mussels were fed on unicellular algae (Chlorella vulgaris) cultured in the laboratory. NP exposures caused significant increases (p < 0.05) in the levels of total glutathione (GSH), reduced-glutathione (rGSH), oxidized-glutathione (GSSG) and malondialdehyde (MDA) in the digestive gland. Oppositely, there were significant (p < 0.05) decreases in acetylcholinesterase activity in the foot muscles. Total energy reserves of the digestive gland and foot muscle significantly (p < 0.05) decreased, but only at the highest NP exposures. Nevertheless, NP exposures did not alter (p > 0.05) the algae filtering capacity of mussels. This study demonstrated that the biomarkers belonging to different metabolic systems responded to NP exposures, suggesting their usage in the monitoring studies for freshwater systems.
在本研究中，研究了淡水贻贝（Unio tigridis）的消化腺和足部肌肉中各种生物标志物在不同浓度（0、1、3和9毫克纳米颗粒/升）下暴露于氧化铝、氧化铜和二氧化钛纳米颗粒14天后的反应。贻贝以实验室培养的单细胞藻类（小球藻）为食。纳米颗粒暴露导致消化腺中总谷胱甘肽（GSH）、还原型谷胱甘肽（rGSH）、氧化型谷胱甘肽（GSSG）和丙二醛（MDA）水平显著升高（p<0.05）。相反，足部肌肉中的乙酰胆碱酯酶活性显著降低（p<0.05）。消化腺和足部肌肉的总能量储备显著降低（p<0.05），但仅在最高纳米颗粒暴露水平下。然而，纳米颗粒暴露并未改变贻贝的藻类过滤能力（p>0.05）。本研究表明，属于不同代谢系统的生物标志物对纳米颗粒暴露有反应，表明它们可用于淡水系统的监测研究。
