Telen M J, Scearce R M, Haynes B F
Vox Sang. 1987;52(3):236-43. doi: 10.1111/j.1423-0410.1987.tb03035.x.
Human erythrocyte membrane proteins express antigens which serve as markers for erythroid differentiation as well as targets for human blood group alloantibodies. We have produced and characterized a new panel of five monoclonal antibodies to erythrocyte membrane proteins. Three monoclonal antibodies (E3, E4, E5) were specific for erythrocyte glycophorins. One antibody (E3) identified the sialoglycoprotein alpha and beta homologous regions proximal to the plasma membrane, a second antibody (E4) was specific for sialoglycoprotein alpha, while a third (E5) was a sialoglycoprotein-beta-specific antibody. Two antibodies (E6 and TE10) to the 65,000-dalton chymotrypsin cleavage product of band 3 were also produced. These antibodies constitute a new panel of probes for investigation of normal erythroid differentiation, erythroleukemia, and the expression of normal and anomalous blood group antigens.
人类红细胞膜蛋白表达的抗原可作为红细胞分化的标志物以及人类血型同种抗体的靶标。我们制备并鉴定了一组针对红细胞膜蛋白的五种新型单克隆抗体。三种单克隆抗体（E3、E4、E5）对红细胞血型糖蛋白具有特异性。一种抗体（E3）识别靠近质膜的唾液酸糖蛋白α和β同源区域，第二种抗体（E4）对唾液酸糖蛋白α具有特异性，而第三种（E5）是唾液酸糖蛋白β特异性抗体。还制备了两种针对带3的65000道尔顿胰凝乳蛋白酶裂解产物的抗体（E6和TE10）。这些抗体构成了一组新的探针，用于研究正常红细胞分化、红白血病以及正常和异常血型抗原的表达。
