Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
Intramural Research Program, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, USA.
Mol Microbiol. 2022 Jan;117(1):10-19. doi: 10.1111/mmi.14842. Epub 2021 Nov 19.
In many bacteria, the stabilities and functions of small regulatory RNAs (sRNAs) that act by base pairing with target RNAs most often are dependent on Hfq or ProQ/FinO-domain proteins, two classes of RNA chaperone proteins. However, while all bacteria appear to have sRNAs, many have neither Hfq nor ProQ/FinO-domain proteins raising the question of whether another factor might act as an sRNA chaperone in these organisms. Several recent studies have reported that KH domain proteins, such as KhpA and KhpB, bind sRNAs. Here we describe what is known about the distribution, structures, RNA-binding properties, and physiologic roles of KhpA and KhpB and discuss evidence for and against these proteins serving as sRNAs chaperones.
在许多细菌中，通过与靶 RNA 碱基配对起作用的小调控 RNA（sRNA）的稳定性和功能通常依赖于 Hfq 或 ProQ/FinO 结构域蛋白这两类 RNA 伴侣蛋白。然而，尽管所有细菌似乎都有 sRNA，但许多细菌既没有 Hfq 也没有 ProQ/FinO 结构域蛋白，这就提出了一个问题，即在这些生物体中是否可能有其他因素充当 sRNA 伴侣。最近的几项研究报告称，KH 结构域蛋白（如 KhpA 和 KhpB）可以结合 sRNA。在这里，我们描述了关于 KhpA 和 KhpB 的分布、结构、RNA 结合特性和生理作用的已知信息，并讨论了这些蛋白作为 sRNA 伴侣的证据。
