Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, Dhaula Kuan, New Delhi, 110021, India.
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400085, India.
Plant J. 2022 Jan;109(1):241-260. doi: 10.1111/tpj.15572. Epub 2021 Nov 26.
Calcium (Ca ) is widely recognized as a key second messenger in mediating various plant adaptive responses. Here we show that calcineurin B-like interacting protein kinase CIPK9 along with its interacting partner VDAC3 identified in the present study are involved in mediating plant responses to methyl viologen (MV). CIPK9 physically interacts with and phosphorylates VDAC3. Co-localization, co-immunoprecipitation, and fluorescence resonance energy transfer experiments proved their physical interaction in planta. Both cipk9 and vdac3 mutants exhibited a tolerant phenotype against MV-induced oxidative stress, which coincided with the lower-level accumulation of reactive oxygen species in their roots. In addition, the analysis of cipk9vdac3 double mutant and VDAC3 overexpressing plants revealed that CIPK9 and VDAC3 were involved in the same pathway for inducing MV-dependent oxidative stress. The response to MV was suppressed by the addition of lanthanum chloride, a non-specific Ca channel blocker indicating the role of Ca in this pathway. Our study suggest that CIPK9-VDAC3 module may act as a key component in mediating oxidative stress responses in Arabidopsis.
钙（Ca）被广泛认为是介导各种植物适应反应的关键第二信使。在这里，我们表明，与本研究中鉴定的相互作用伙伴 VDAC3 相互作用的钙调神经磷酸酶 B 样相互作用蛋白激酶 CIPK9 参与介导植物对甲基紫精（MV）的反应。CIPK9 与 VDAC3 相互作用并磷酸化 VDAC3。共定位、共免疫沉淀和荧光共振能量转移实验证明了它们在植物体内的物理相互作用。cipk9 和 vdac3 突变体对 MV 诱导的氧化应激表现出耐受性表型，这与它们根部活性氧的积累水平较低相一致。此外，对 cipk9vdac3 双突变体和 VDAC3 过表达植物的分析表明，CIPK9 和 VDAC3 参与了诱导 MV 依赖性氧化应激的相同途径。添加镧氯化物（一种非特异性 Ca 通道阻滞剂）抑制了对 MV 的反应，表明 Ca 在该途径中的作用。我们的研究表明，CIPK9-VDAC3 模块可能作为介导拟南芥氧化应激反应的关键组成部分。
