群体结构、遗传多样性以及适应环境胁迫的候选基因于……中

Population Structure, Genetic Diversity and Candidate Genes for the Adaptation to Environmental Stress in .

作者信息

Wang Ya, Jiang Zeping, Qin Aili, Wang Fude, Chang Ermei, Liu Yifu, Nie Wen, Tan Cancan, Yuan Yanchao, Dong Yao, Huang Ruizhi, Jia Zirui, Wang Junhui

机构信息

State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

Key Laboratory of Forest Ecology and Environment of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China.

出版信息

Plants (Basel). 2023 Mar 10;12(6):1266. doi: 10.3390/plants12061266.

DOI:10.3390/plants12061266

PMID:36986954

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10055018/

Abstract

is major silvicultural and timber species in northeast China, and its distribution area is an important transition zone for genus spruce migration. The degree of intraspecific differentiation of is high, but population structure and differentiation mechanisms are not clear. In this study, 523,761 single nucleotide polymorphisms (SNPs) were identified in 113 individuals from 9 populations of by genotyping-by-sequencing (GBS). Population genomic analysis showed that was divided into three geoclimatic regions: Great Khingan Mountains climatic region, Lesser Khingan Mountains climatic region, and Changbai Mountain climatic region. Mengkeshan (MKS) population on the northern edge of the distribution area and Wuyiling (WYL) population located in the mining area are two highly differentiated groups. Selective sweep analysis showed that MKS and WYL populations had 645 and 1126 selected genes, respectively. Genes selected in the MKS population were associated with flowering and photomorphogenesis, cellular response to water deficit, and glycerophospholipid metabolism; genes selected in the WYL population were associated with metal ion transport, biosynthesis of macromolecules, and DNA repair. Climatic factors and heavy metal stress drives divergence in MKS and WYL populations, respectively. Our findings provide insights into adaptive divergence mechanisms in and will contribute to molecular breeding studies.

摘要

是中国东北地区主要的造林和木材树种，其分布区域是云杉属迁移的重要过渡地带。该树种的种内分化程度较高，但种群结构和分化机制尚不清楚。在本研究中，通过简化基因组测序（GBS）对9个种群的113个个体进行基因分型，在该树种中鉴定出523,761个单核苷酸多态性（SNP）。群体基因组分析表明，该树种可分为三个地理气候区域：大兴安岭气候区、小兴安岭气候区和长白山气候区。分布区北缘的孟克山（MKS）种群和位于矿区的五营（WYL）种群是两个高度分化的群体。选择性清除分析表明，MKS和WYL种群分别有645个和1126个选择基因。MKS种群中选择的基因与开花和光形态建成、对水分亏缺的细胞反应以及甘油磷脂代谢有关；WYL种群中选择的基因与金属离子转运、大分子生物合成和DNA修复有关。气候因子和重金属胁迫分别驱动了MKS和WYL种群的分化。我们的研究结果为该树种的适应性分化机制提供了见解，并将有助于分子育种研究。