School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou 341000, China.
Langmuir. 2021 Nov 23;37(46):13757-13766. doi: 10.1021/acs.langmuir.1c02528. Epub 2021 Nov 8.
Nanoemulsions have become extremely popular water-insoluble pesticide delivery systems in recent years. In this study, prochloraz nanoemulsions were obtained by selecting the mixing ratio of surfactants (6:1, 3:1, 2:1, 1:1, 1:2, 1:3, and 1:6), surfactant concentration, and shearing time. The optimal formula was 10 wt % prochloraz, 6 wt % surfactant (2 wt % CO-100 + 4 wt % CO-360) dissolved in 6 wt % hydrocarbon solvent (S-100A), and deionized water replenished to 100 wt %. This formula meets the quality index standards of the Food and Agriculture Organization. Compared with oil-in-water emulsion (EW), the prochloraz nanoemulsion exhibited higher antifungal activity against in vitro (lower LC of 1.17 mg L) and in vivo (fewer lesions). In addition, the L02 cells treated with the nanoemulsion had a higher survival rate and lower apoptosis rate at the same concentration. Results showed that the toxicity of the prochloraz nanoemulsion on L02 cells was lower than that of EW. The findings provide an important method for developing an efficient, safe, and environment-friendly nanoemulsion for postharvest fruit storage.
近年来，纳米乳剂已成为极受欢迎的水不溶性农药传递系统。在这项研究中，通过选择表面活性剂（6:1、3:1、2:1、1:1、1:2、1:3 和 1:6）的混合比、表面活性剂浓度和剪切时间，获得了百菌清纳米乳剂。最佳配方为 10wt%百菌清、6wt%表面活性剂（2wt%CO-100+4wt%CO-360）溶解于 6wt%烃类溶剂（S-100A），并用去离子水补充至 100wt%。该配方符合粮农组织的质量指标标准。与水乳剂（EW）相比，百菌清纳米乳剂对 的体外（LC 更低，为 1.17mg L）和体内（病斑更少）均表现出更高的抗真菌活性。此外，在相同浓度下，用纳米乳剂处理的 L02 细胞具有更高的存活率和更低的凋亡率。结果表明，百菌清纳米乳剂对 L02 细胞的毒性低于 EW。这些发现为开发高效、安全、环保的采后水果贮藏纳米乳剂提供了重要方法。
