Li Yi, Zhao Runan, Li Yan, Zhou Zhiqin
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.
Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China.
Microorganisms. 2021 Apr 30;9(5):969. doi: 10.3390/microorganisms9050969.
, the cause of citrus blue mold, is a pathogenic fungus that seriously affects the postharvest quality of citrus fruit and causes serious economic loss. In this study, a eugenol nanoemulsion containing limonin, an antimicrobial component from citrus seeds, was prepared using a high-pressure microfluidizer and the antifungal activity of the nanoemulsions against was evaluated based on the conidial germination rate, mycelial growth, and scanning electron microscopy analysis. The results showed that the minimum inhibitory concentration and the inhibition rate of limonin-loaded eugenol nanoemulsion was 160 μg/mL and 59.21%, respectively, which was more potent than that of the limonin-free eugenol emulsion. After treatment with the nanoemulsions, the integrity of the cell membrane was disrupted, the cell morphology was abnormal, and the leakage of nucleic acid and protein was observed. In addition, the challenge test on citrus fruits revealed that the limonin-loaded eugenol emulsion inhibited citrus infection for longer periods, with an infection rate of 29.2% after 5 days. The current research shows that nanoemulsions containing limonin and eugenol have effective antifungal activity against , and may be used as a substitute for inhibiting blue mold in citrus fruits.
柑橘青霉，是一种严重影响柑橘类水果采后品质并造成严重经济损失的致病真菌。在本研究中，使用高压微射流制备了一种含有柠檬苦素（一种来自柑橘种子的抗菌成分）的丁香酚纳米乳液，并基于分生孢子萌发率、菌丝生长和扫描电子显微镜分析评估了该纳米乳液对柑橘青霉的抗真菌活性。结果表明，负载柠檬苦素的丁香酚纳米乳液的最低抑菌浓度和抑菌率分别为160μg/mL和59.21%，比不含柠檬苦素的丁香酚乳液更有效。用纳米乳液处理后，柑橘青霉细胞膜的完整性被破坏，细胞形态异常，并且观察到核酸和蛋白质泄漏。此外，对柑橘果实的挑战试验表明，负载柠檬苦素的丁香酚乳液抑制柑橘感染的时间更长，5天后感染率为29.2%。目前的研究表明，含有柠檬苦素和丁香酚的纳米乳液对柑橘青霉具有有效的抗真菌活性，并且可作为抑制柑橘类水果青霉病的替代品。
