key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery Huazhong Agricultural University, Wuhan 430070, China.
Guangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning 530021, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Mar;253:109212. doi: 10.1016/j.cbpc.2021.109212. Epub 2021 Nov 5.
Nitrite stress is a major environmental factor that limits aquatic animal growth, reproduction and survival. Even so, some shrimps still can withstand somewhat high concentrations of nitrite environment. However, few studies have been conducted about the tolerance molecular mechanism of Litopenaeus vannamei in the high concentration nitrite. To identify the genes and pathways involved in the regulation of nitrite tolerance, we performed comparative transcriptomic analysis in the L. vannamei nitrite-tolerant (NT) and nitrite-sensitive (NS) families, and untreated shrimps were used as the control group. After 24 h of nitrite exposure (NaNO, 112.5 mg/L), a total of 1521 and 868 differentially expressed genes (DEGs) were obtained from NT compared with NS and control group, respectively. Functional enrichment analysis revealed that most of these DEGs were involved in immune defense, energy metabolism processes and endoplasmic reticulum (ER) stress. During nitrite stress, energy metabolism in NT was significantly enhanced by activating the related genes expression of oxidative phosphorylation (OXPHOS) pathway and tricarboxylic acid (TCA) cycle. Meanwhile, some DEGs involved in innate immunity- related genes and pathways, and ER stress responses also were highly expressed in NT. Therefore, we speculate that accelerated energy metabolism, higher expression of immunity and ER related genes might be the important adaptive strategies for NT in relative to NS under nitrite stress. These results will provide new insights on the potential tolerant molecular mechanisms and the breeding of new varieties of nitrite tolerant L. vannamei.
亚硝酸盐胁迫是限制水生动物生长、繁殖和生存的主要环境因素。尽管如此，一些虾类仍然能够承受一定浓度的亚硝酸盐环境。然而，关于凡纳滨对虾在高浓度亚硝酸盐下的耐受力分子机制的研究较少。为了鉴定参与调控耐亚硝酸盐能力的基因和途径，我们对凡纳滨对虾耐亚硝酸盐（NT）和敏感（NS）家系进行了比较转录组分析，并以未经处理的虾作为对照组。在亚硝酸盐暴露 24 小时后（NaNO₂，112.5mg/L），从 NT 与 NS 组相比，以及与对照组相比，分别获得了 1521 个和 868 个差异表达基因（DEGs）。功能富集分析表明，这些 DEGs 大多数参与免疫防御、能量代谢过程和内质网（ER）应激。在亚硝酸盐胁迫下，NT 通过激活氧化磷酸化（OXPHOS）途径和三羧酸（TCA）循环的相关基因表达，显著增强了能量代谢。同时，NT 中一些与先天免疫相关的基因和途径以及 ER 应激反应的 DEGs 也高度表达。因此，我们推测在亚硝酸盐胁迫下，相对于 NS，加速的能量代谢、更高表达的免疫和 ER 相关基因可能是 NT 的重要适应策略。这些结果将为耐亚硝酸盐凡纳滨对虾的潜在耐受力分子机制和新品种的培育提供新的见解。
