Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery Huazhong Agricultural University, Wuhan, PR China.
Guangxi Academy of Fishery Sciences, GuangxiKey Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Nanning 530021, PR China.
Sci Total Environ. 2020 Apr 1;711:134416. doi: 10.1016/j.scitotenv.2019.134416. Epub 2019 Sep 12.
Nitrite accumulation in aquatic environments is a potential risk factor that disrupts multiple physiological functions in aquatic animals. In this study, the physiology, transcriptome and metabolome of the control group (LV-C), nitrite-tolerance group (LV-NT) and nitrite-sensitive group (LV-NS) were investigated to identify the stress responses and mechanisms underlying the nitrite tolerance of Litopenaeus vannamei. After LV-NT and LV-NS were subjected to nitrite stress, the hemocyanin contents were significantly decreased, and hepatopancreas showed severe histological damage compared with LV-C. Likewise, the antioxidant enzymes were also significantly changed after nitrite exposure. The transcriptome data revealed differentially expressed genes associated with immune system, cytoskeleton remodeling and apoptosis in LV-NT and LV-NS. The combination of transcriptomic and metabolomic analysis revealed nitrite exposure disturbed metabolism processes in L. vannamei, including amino acid metabolism, nucleotide metabolism and lipid metabolism. The multiple comparative analysis implicated that higher nitrite tolerance of LV-NT than LV-NS may be attributed to enhanced hypoxia inducible factor-1α expression to regulate energy supply and gaseous exchange. Moreover, LV-NT showed higher antioxidative ability, detoxification gene expression and enhanced fatty acids contents after nitrite exposure in relative to LV-NS. Collectively, all these results will greatly provide new insights into the molecular mechanisms underlying the stress responses and tolerance of nitrite exposure in L. vannamei.
在水生环境中，亚硝酸盐的积累是一种潜在的风险因素，会破坏水生动物的多种生理功能。在这项研究中，我们研究了对照组（LV-C）、亚硝酸盐耐受组（LV-NT）和亚硝酸盐敏感组（LV-NS）的生理学、转录组和代谢组，以确定凡纳滨对虾对亚硝酸盐耐受的应激反应和机制。在 LV-NT 和 LV-NS 受到亚硝酸盐胁迫后，血蓝蛋白含量显著降低，与 LV-C 相比，肝胰腺显示出严重的组织学损伤。同样，抗氧化酶在暴露于亚硝酸盐后也发生了显著变化。转录组数据显示，LV-NT 和 LV-NS 中与免疫系统、细胞骨架重塑和细胞凋亡相关的差异表达基因。转录组和代谢组联合分析表明，亚硝酸盐暴露扰乱了凡纳滨对虾的代谢过程，包括氨基酸代谢、核苷酸代谢和脂质代谢。多重比较分析表明，LV-NT 比 LV-NS 具有更高的耐亚硝酸盐能力，这可能归因于增强缺氧诱导因子-1α的表达，以调节能量供应和气体交换。此外，与 LV-NS 相比，LV-NT 在暴露于亚硝酸盐后表现出更高的抗氧化能力、解毒基因表达和增强的脂肪酸含量。总的来说，这些结果将极大地提供对凡纳滨对虾应激反应和耐亚硝酸盐暴露的分子机制的新见解。
