State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Bioresour Technol. 2022 Jan;344(Pt B):126298. doi: 10.1016/j.biortech.2021.126298. Epub 2021 Nov 5.
A pretreatment method combining ball-milling, ultrasound, and hydrothermal treatment was developed to produce xylooligosaccharides (XOS) and glucose with a high yield from corn stover. Under optimal conditions, the yield of XOS reached 80.40%, and the functional XOS (X2-X4) took up to 26.97%. Small amount of inhibitors were formed during the hydrothermal process. Enzymatic hydrolysis of the hydrothermally pretreated residue gave 92.60% yield of glucose, leaving lignin as the final residue which accounted for 66.82% of native lignin. The correlations between the yield of glucose and the physio-chemical properties of corn stover, such as crystalline index, particle size, and the removal of xylan, were established to understand the recalcitrance removal during the pretreatment process. Results demonstrate that this combined pretreatment method is a green and effective process to selectively separate the hemicellulose fractions and improve both production of XOS and glucose yield.
采用球磨、超声和水热处理相结合的预处理方法，从玉米秸秆中高产制备木低聚糖（XOS）和葡萄糖。在最佳条件下，XOS 的产率达到 80.40%，功能性 XOS（X2-X4）占比高达 26.97%。水热过程中会形成少量抑制剂。对水热预处理残渣进行酶解，可得到 92.60%的葡萄糖产率，木质素作为最终残渣，占原木质素的 66.82%。建立了葡萄糖产率与玉米秸秆物理化学性质（如结晶指数、粒径和木聚糖去除率）之间的相关性，以了解预处理过程中对木质素的去除情况。结果表明，该组合预处理方法是一种绿色有效的工艺，可选择性分离半纤维素组分，提高 XOS 和葡萄糖的产量。
