Zhang Fulong, Lan Wu, Li Zengyong, Zhang Aiping, Tang Baoling, Wang Huihui, Wang Xiaoying, Ren Junli, Liu Chuanfu
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Bioresour Technol. 2021 Oct;337:125327. doi: 10.1016/j.biortech.2021.125327. Epub 2021 May 24.
The aim of this work was to develop a feasible ball mill-assisted alkaline peroxide pretreatment followed by stepwise hydrolysis to improve the yield of xylo-oligosaccharides (XOS) and fermentable sugars. The hydrogen peroxide charge, ball-milling time, and solid-to-liquid ratio affected the compositions, particle sizes, morphology, and crystallinity of the corn stover, directly improving the following hydrolytic efficiency. The optimal pretreatment was with 0.45 g/g (HO: substrate) and 1:3 solid-to-liquid ratio (w/v) for 1.0 h ball-milling, resulting in 84.29% delignification. Physicochemical properties of the pretreated samples were characterized and their correlations to the enzymatic hydrolysis were revealed. Compared with one-step cellulase hydrolysis, the two-step xylanase-cellulase hydrolysis of the pretreated corn stover showed significant advance in preparing XOS, producing 69.65% (on the base of xylan content in pretreated sample) of XOS, along with 20.55% xylose, 68.94% glucose, and 21.15% gluco-oligosaccharides. The yield of XOS was 2-7 times higher than those in previous studies.
这项工作的目的是开发一种可行的球磨辅助碱性过氧化氢预处理方法，随后进行分步水解，以提高木寡糖（XOS）和可发酵糖的产量。过氧化氢用量、球磨时间和固液比对玉米秸秆的组成、粒径、形态和结晶度有影响，直接提高了后续的水解效率。最佳预处理条件为0.45 g/g（H₂O₂:底物）、1:3的固液比（w/v），球磨1.0小时，脱木素率达84.29%。对预处理样品的物理化学性质进行了表征，并揭示了它们与酶水解的相关性。与一步纤维素酶水解相比，预处理玉米秸秆的两步木聚糖酶-纤维素酶水解在制备XOS方面有显著进展，产生了69.65%（基于预处理样品中的木聚糖含量）的XOS，以及20.55%的木糖、68.94%的葡萄糖和21.15%的葡萄糖寡糖。XOS的产量比以前的研究高出2至7倍。
