对挪威奶山羊血液中的基因变异进行搜索后，发现血红蛋白βA位点存在一种新的多态性。

Search for genetic variation in the blood of Norwegian dairy goats reveals a new polymorphism at the Hb beta A locus.

作者信息

Braend M, Tucker E M, Clarke S W

出版信息

Anim Genet. 1987;18(1):75-9. doi: 10.1111/j.1365-2052.1987.tb00746.x.

DOI:10.1111/j.1365-2052.1987.tb00746.x

Abstract

A total of 150 blood samples tested for serum albumin and transferrin and for red cell carbonic anhydrase, phosphoglucomutase, phosphogluconate dehydrogenase, phosphohexose isomerase, nucleoside phosphorylase, acid phosphatase, 'X'-protein and potassium concentration only showed variation at the 'X' protein and nucleoside phosphorylase loci. Isoelectric focusing over pH range 6-8 showed 145 samples to be of haemoglobin type A and 5 type AD. The haemoglobin A type was resolved into further types by separation over pH 6.9-7.5 in Immobiline polyacrylamide gels. A 2- or 4-band pattern was present in 136 of the samples; a genetic hypothesis based on four or more different haemoglobin A variants is proposed. 14 samples had a 3-, 5- or 6-band pattern. It is assumed that these are heterozygous for a variant of the II alpha gene.

摘要

总共150份血液样本检测了血清白蛋白、转铁蛋白、红细胞碳酸酐酶、磷酸葡萄糖变位酶、磷酸葡萄糖酸脱氢酶、磷酸己糖异构酶、核苷磷酸化酶、酸性磷酸酶、“X”蛋白和钾浓度，结果仅显示在“X”蛋白和核苷磷酸化酶位点存在变异。在pH值6 - 8范围内的等电聚焦显示，145份样本为血红蛋白A型，5份为AD型。通过在Immobiline聚丙烯酰胺凝胶上于pH 6.9 - 7.5范围内进行分离，血红蛋白A型进一步分为不同类型。136份样本呈现2条或4条带的模式；提出了基于四种或更多不同血红蛋白A变体的遗传假说。14份样本呈现3条、5条或6条带的模式。假定这些样本是IIα基因变体的杂合子。