State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198 Shiqiao Road, Hangzhou 310021, PR China; Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Hangzhou 310021, PR China; Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Hangzhou 310021, PR China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198 Shiqiao Road, Hangzhou 310021, PR China.
J Chromatogr A. 2021 Dec 6;1659:462589. doi: 10.1016/j.chroma.2021.462589. Epub 2021 Oct 20.
An integrated QuEChERS strategy was developed by combining the extraction and purification processes into a single step. All of the pretreatment procedures could be performed in one tube within 5 min with the aid of magnetic nanoparticles and careful optimization of the key parameters, including the dosages of the sorbents (magnetic nanoparticles, C18, and graphitized carbon black), dehydrating and salting out reagents. The optimal method was validated and compared with the conventional QuEChERS method, demonstrating its clear superiority in terms of operating procedure, sample pretreatment time, and reagent dosages while affording equivalent pesticide recoveries and matrix effects. Further application of this method was performed to analyze 127 pesticide residues in solanaceous vegetables (tomato, pepper, and eggplant), leafy vegetables (brassica campestris and cabbage), legumes (green beans and cowpea), melon-type vegetables (cucumber and towel gourd), and a root vegetable (water bamboo), with the mean recoveries of the pesticides in the individual vegetable samples ranging from 70.6 to 92.8%. The method LOQs for these pesticides ranged from 10 to 50 μg/kg depending on the matrix. These results fully confirmed its wide applicability and versatility for achieving robust, rapid, and high-throughput multi-pesticide residues analysis in vegetable samples. More importantly, the developed strategy provides a greener and more "QuEChERS" design concept, which could be applied to the analysis of numerous types of pesticide residues in various matrices.
建立了一种集成的 QuEChERS 策略，通过将提取和净化过程合并为一个步骤来实现。所有的预处理程序都可以在 5 分钟内，借助磁性纳米粒子并对关键参数（包括吸附剂（磁性纳米粒子、C18 和石墨化炭黑）、脱水和盐析试剂的用量）进行仔细优化，在一个管内完成。对最佳方法进行了验证，并与常规 QuEChERS 方法进行了比较，证明其在操作程序、样品预处理时间和试剂用量方面具有明显优势，同时获得了相当的农药回收率和基质效应。进一步将该方法应用于分析茄科蔬菜（番茄、辣椒和茄子）、叶菜类（白菜和甘蓝）、豆类（青豆和豇豆）、瓜类蔬菜（黄瓜和丝瓜）和根茎类蔬菜（茭白）中的 127 种农药残留，各蔬菜样品中农药的平均回收率在 70.6%至 92.8%之间。这些农药在各基质中的方法 LOQs 为 10 至 50μg/kg 不等。这些结果充分证实了该方法在蔬菜样品中实现稳健、快速和高通量多农药残留分析的广泛适用性和多功能性。更重要的是，所开发的策略提供了一种更绿色、更“QuEChERS”的设计理念，可应用于各种基质中多种类型农药残留的分析。
