Scheper T, Makryaleas K, Nowottny C, Likidis Z, Tsikas D, Schügerl K
Ann N Y Acad Sci. 1987;501:165-70. doi: 10.1111/j.1749-6632.1987.tb45702.x.
Liquid membrane reactors are well known for metal extraction. This technology may also be applied to the immobilization of enzymes in enzyme emulsions. The use of liquid membrane reactors for enzymatic bioconversions has several advantages in comparison to solid membrane reactors and conventional immobilization techniques: there is no membrane fouling, enzyme emulsions can be used in cell-free fermentation broths, in complex mixtures the membrane can preselect the desired substrate for enzymatic reaction, and substances that might decrease the enzyme activity can be excluded. The separation effect is not based on differences in molecular weight but on the chemical behavior of the substances to be separated. Thus, it is not necessary to use cofactors with increased molecular weight for enzymatic reactions, since the coenzyme cannot permeate the liquid membrane. The three systems presented here indicate that enzyme systems can be easily immobilized in liquid surfactant membrane emulsions and there is a broad field of application for enzyme emulsions.
液膜反应器在金属萃取方面广为人知。这项技术也可应用于将酶固定在酶乳液中。与固体膜反应器和传统固定技术相比，使用液膜反应器进行酶促生物转化具有几个优点：不存在膜污染，酶乳液可用于无细胞发酵液，在复杂混合物中，膜可预先选择用于酶促反应的所需底物，并且可能降低酶活性的物质可被排除。分离效果不是基于分子量的差异，而是基于待分离物质的化学行为。因此，酶促反应无需使用分子量增加的辅因子，因为辅酶不能透过液膜。这里介绍的三个系统表明，酶系统可轻松固定在液体表面活性剂膜乳液中，并且酶乳液有广阔的应用领域。
